PMID- 10445160
OWN - NLM
STAT- MEDLINE
DCOM- 19991013
LR  - 20190116
IS  - 0148-0545 (Print)
IS  - 0148-0545 (Linking)
VI  - 22
IP  - 3
DP  - 1999 Aug
TI  - Contact sensitivity to selected acrylate compounds in B6C3F1 mice: relative 
      potency, cross reactivity, and comparison of test methods.
PG  - 491-506
AB  - Given the increasing prevalence of occupational sensitization to acrylate 
      compounds, n-butyl acrylate (BAC), ethyl acrylate (EAC), and trimethylol propane 
      triacrylate (TMT) were recommended by the National Toxicology Program for 
      hypersensitivity testing in female B6C3F1 mice. The objectives of these studies 
      were to determine the irritating and sensitizing potential of these three 
      compounds using an irritation assay, the murine Local Lymph Node Assay (LLNA), 
      and the Mouse Ear Swelling Test (MEST). The minimal irritating concentration for 
      TMT was determined to be 1.0%, whereas BAC and EAC demonstrated no irritation up 
      to 30%, the highest concentration tested. TMT tested positive in the LLNA at 
      concentrations as low as 0.1% whereas an induction concentration of 0.3% was 
      required to elicit a positive response in the MEST. Furthermore, BAC tested 
      negative in the MEST at induction concentrations as high as 30%, but yielded 
      positive results in the LLNA at concentrations as low as 20%. EAC, at all 
      concentrations tested, was negative in both the MEST and the LLNA. Cross 
      reactivity was only seen when mice were sensitized with TMT and challenged with 
      BAC. In these studies, the LLNA was a more sensitive indicator of the allergic 
      potential of these three acrylates when compared to the MEST.
FAU - Hayes, B B
AU  - Hayes BB
AD  - National Institute for Occupational Safety and Health, Health Effects Laboratory 
      Division, Morgantown, WV 26505, USA.
FAU - Meade, B J
AU  - Meade BJ
LA  - eng
GR  - ES05288/ES/NIEHS NIH HHS/United States
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Drug Chem Toxicol
JT  - Drug and chemical toxicology
JID - 7801723
RN  - 0 (Acrylates)
SB  - IM
MH  - Acrylates/*toxicity
MH  - Animals
MH  - Cell Division/drug effects
MH  - Cross Reactions
MH  - Dermatitis, Contact/*etiology
MH  - Female
MH  - Lymph Nodes/drug effects/pathology
MH  - Mice
MH  - Mice, Inbred C3H
MH  - Mice, Inbred C57BL
MH  - Toxicology/methods
EDAT- 1999/08/13 00:00
MHDA- 1999/08/13 00:01
CRDT- 1999/08/13 00:00
PHST- 1999/08/13 00:00 [pubmed]
PHST- 1999/08/13 00:01 [medline]
PHST- 1999/08/13 00:00 [entrez]
AID - 10.3109/01480549909042528 [doi]
PST - ppublish
SO  - Drug Chem Toxicol. 1999 Aug;22(3):491-506. doi: 10.3109/01480549909042528.