PMID- 10445843
OWN - NLM
STAT- MEDLINE
DCOM- 19990907
LR  - 20201209
IS  - 0950-9232 (Print)
IS  - 0950-9232 (Linking)
VI  - 18
IP  - 26
DP  - 1999 Jul 1
TI  - A human homolog of Drosophila lethal(3)malignant brain tumor (l(3)mbt) protein 
      associates with condensed mitotic chromosomes.
PG  - 3799-809
AB  - The lethal(3)malignant brain tumor (D-l(3)mbt) gene is considered to be one of 
      the tumor suppressor genes of Drosophila, and its recessive mutations are 
      associated with malignant transformation of the neuroblasts in the larval brain. 
      The structure of D-l(3)mbt protein is similar to Drosophila sex comb on midleg 
      (Scm) protein which is a member of Polycomb group (PcG) proteins. We have 
      isolated here the first human homolog of the D-l(3)mbt gene, designated 
      h-l(3)mbt. Radiation hybrid mapping and fluorescence in situ hybridization (FISH) 
      analysis localized the h-l(3)mbt gene to chromosome 20q12. The h-l(3)mbt 
      transcript is expressed in most of the human adult normal tissues and cultured 
      cell lines. However, some cancer cells markedly reduce the h-l(3)mbt protein 
      expression. Immunocytochemical study revealed that the h-l(3)mbt protein shows a 
      speckled and scattered distribution in interphase nuclei and completely 
      associates with condensed chromosomes in mitotic cells. This subcellular 
      localization has been shown to be different from that of Bmi1 protein which is a 
      component of PcG complex. Furthermore, overexpression of h-l(3)mbt protein by 
      using a Cre-mediated gene activation system leads to failures of proper 
      chromosome segregation and cytokinesis, which result in formation of multinuclei 
      in U251MG cells. These observations suggest that h-l(3)mbt protein has functions 
      distinct from those of PcG proteins and may play a role in proper progression of 
      cell division.
FAU - Koga, H
AU  - Koga H
AD  - Department of Tumor Genetics and Biology, Kumamoto University School of Medicine, 
      Japan.
FAU - Matsui, S
AU  - Matsui S
FAU - Hirota, T
AU  - Hirota T
FAU - Takebayashi, S
AU  - Takebayashi S
FAU - Okumura, K
AU  - Okumura K
FAU - Saya, H
AU  - Saya H
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Oncogene
JT  - Oncogene
JID - 8711562
RN  - 0 (Chromosomal Proteins, Non-Histone)
RN  - 0 (L3MBTL1 protein, human)
RN  - 0 (Neoplasm Proteins)
RN  - 0 (Repressor Proteins)
RN  - 0 (Tumor Suppressor Proteins)
SB  - IM
MH  - Adult
MH  - Amino Acid Sequence
MH  - Animals
MH  - Brain Neoplasms/genetics/pathology
MH  - Cell Division/drug effects
MH  - Cell Line
MH  - Chromosomal Proteins, Non-Histone
MH  - Chromosomes, Human/*metabolism
MH  - Chromosomes, Human, Pair 20/*genetics
MH  - Drosophila melanogaster/*genetics
MH  - Expressed Sequence Tags
MH  - Gene Expression Regulation
MH  - Genes, Recessive
MH  - *Genes, Tumor Suppressor
MH  - Glioma/genetics/pathology
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Interphase
MH  - *Mitosis
MH  - Molecular Sequence Data
MH  - Neoplasm Proteins/deficiency/genetics/*metabolism
MH  - Polymerase Chain Reaction
MH  - Rats
MH  - Repressor Proteins
MH  - Sequence Alignment
MH  - Sequence Homology, Amino Acid
MH  - Species Specificity
MH  - Subcellular Fractions
MH  - Transcriptional Activation
MH  - Tumor Cells, Cultured
MH  - Tumor Suppressor Proteins
EDAT- 1999/08/13 00:00
MHDA- 1999/08/13 00:01
CRDT- 1999/08/13 00:00
PHST- 1999/08/13 00:00 [pubmed]
PHST- 1999/08/13 00:01 [medline]
PHST- 1999/08/13 00:00 [entrez]
AID - 10.1038/sj.onc.1202732 [doi]
PST - ppublish
SO  - Oncogene. 1999 Jul 1;18(26):3799-809. doi: 10.1038/sj.onc.1202732.