PMID- 10446295
OWN - NLM
STAT- MEDLINE
DCOM- 19990928
LR  - 20190610
IS  - 0006-3002 (Print)
IS  - 0006-3002 (Linking)
VI  - 1420
IP  - 1-2
DP  - 1999 Aug 20
TI  - TrkB mutant lacking the amino-terminal half of the extracellular portion acts as 
      a functional brain-derived neurotrophic factor receptor.
PG  - 104-10
AB  - A series of mutants with deletion in the extracellular portion of TrkB were 
      expressed transiently and stably in mammalian cells to examine the brain-derived 
      neurotrophic factor (BDNF)-binding properties of TrkB. We found that these 
      binding activities were retained by the TrkB deletion mutant (TrkBDelta4) lacking 
      most of the extracellular portion, cysteine-rich cluster 1 and 2, leucine-rich 
      motif and most of the first immunoglobulin-like domain (Ig1). Furthermore, the 
      results of the neurotrophin selectivity, the equilibrium binding constant, 
      auto-phosphorylation and BDNF dependent cell survival indicate that TrkBDelta4 
      acts as a functional BDNF receptor comparable to wild-type TrkB. Thus, our 
      findings showed that only the carboxyl-terminal half of the extracellular portion 
      of TrkB, which includes the Ig2 domain, is essential for the functional BDNF 
      receptor.
FAU - Kojima, S
AU  - Kojima S
AD  - Shionogi Institute for Medical Science, 2-5-1, Mishima, Settsu-shi, Osaka 
      566-0022, Japan.
FAU - Nakayama, T
AU  - Nakayama T
FAU - Kuwajima, G
AU  - Kuwajima G
FAU - Suzuki, H
AU  - Suzuki H
FAU - Sakata, T
AU  - Sakata T
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PL  - Netherlands
TA  - Biochim Biophys Acta
JT  - Biochimica et biophysica acta
JID - 0217513
RN  - 0 (Brain-Derived Neurotrophic Factor)
RN  - 0 (Peptide Fragments)
RN  - 0 (Receptor, Ciliary Neurotrophic Factor)
RN  - 0 (Receptors, Nerve Growth Factor)
RN  - 0 (Recombinant Proteins)
RN  - EC 2.7.10.1 (Receptor Protein-Tyrosine Kinases)
SB  - IM
MH  - 3T3 Cells
MH  - Animals
MH  - Binding Sites/genetics
MH  - Brain-Derived Neurotrophic Factor/*metabolism
MH  - COS Cells
MH  - In Vitro Techniques
MH  - Mice
MH  - Peptide Fragments/chemistry/*genetics/*metabolism
MH  - Protein Conformation
MH  - Receptor Protein-Tyrosine Kinases/chemistry/*genetics/*metabolism
MH  - Receptor, Ciliary Neurotrophic Factor
MH  - Receptors, Nerve Growth Factor/chemistry/*genetics/*metabolism
MH  - Recombinant Proteins/chemistry/genetics/metabolism
MH  - Sequence Deletion
EDAT- 1999/08/14 00:00
MHDA- 1999/08/14 00:01
CRDT- 1999/08/14 00:00
PHST- 1999/08/14 00:00 [pubmed]
PHST- 1999/08/14 00:01 [medline]
PHST- 1999/08/14 00:00 [entrez]
AID - S0005-2736(99)00094-2 [pii]
AID - 10.1016/s0005-2736(99)00094-2 [doi]
PST - ppublish
SO  - Biochim Biophys Acta. 1999 Aug 20;1420(1-2):104-10. doi: 
      10.1016/s0005-2736(99)00094-2.