PMID- 10447202
OWN - NLM
STAT- MEDLINE
DCOM- 19990923
LR  - 20161124
IS  - 0739-1102 (Print)
IS  - 0739-1102 (Linking)
VI  - 16
IP  - 6
DP  - 1999 Jun
TI  - Sequence specific hybridization properties of methylphosphonate 
      oligodeoxynucleotides.
PG  - 1177-88
AB  - Methylphosphonate oligodeoxynucleotides (MPO's) with isomerically pure 
      Rp-configurated methylphosphonates (MP's) were synthesized by block coupling of 
      ApT and TpA dinucleoside methylphosphonates (DMP's, p indicating MP-linkage). 
      Oligonucleotide duplexes (20 mers) with these Rp-MP's showed almost the same 
      melting temperatures (Tm) as those with phosphorodiester bonds. Further a 
      dependence of the duplex stability from the nucleosides (bases) adjacent to the 
      MP moiety was observed. For the first time thermodynamic parameters for the 
      duplex to coil transition of isomerically pure MP's were determined from the 
      concentration dependence of the Tm. CD-spectra of the duplexes show structural 
      changes which can be associated with the transition to a compact helix with 
      higher helix winding angle.
FAU - Schweitzer, M
AU  - Schweitzer M
AD  - Institute of Organic Chemistry, Johann Wolfgang Goethe-Universitat, Frankfurt am 
      Main, Germany.
FAU - Engels, J W
AU  - Engels JW
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - J Biomol Struct Dyn
JT  - Journal of biomolecular structure & dynamics
JID - 8404176
RN  - 0 (Nucleic Acid Heteroduplexes)
RN  - 0 (Oligodeoxyribonucleotides)
RN  - 0 (Organophosphorus Compounds)
RN  - 329W4YM10Z (methylphosphonic acid)
SB  - IM
MH  - Nucleic Acid Conformation
MH  - Nucleic Acid Heteroduplexes
MH  - Nucleic Acid Hybridization
MH  - Oligodeoxyribonucleotides/*chemistry
MH  - Organophosphorus Compounds/*chemistry
MH  - Static Electricity
EDAT- 1999/08/14 00:00
MHDA- 1999/08/14 00:01
CRDT- 1999/08/14 00:00
PHST- 1999/08/14 00:00 [pubmed]
PHST- 1999/08/14 00:01 [medline]
PHST- 1999/08/14 00:00 [entrez]
AID - 10.1080/07391102.1999.10508326 [doi]
PST - ppublish
SO  - J Biomol Struct Dyn. 1999 Jun;16(6):1177-88. doi: 10.1080/07391102.1999.10508326.