PMID- 10447750
OWN - NLM
STAT- MEDLINE
DCOM- 20000404
LR  - 20190513
IS  - 0019-2805 (Print)
IS  - 1365-2567 (Electronic)
IS  - 0019-2805 (Linking)
VI  - 97
IP  - 2
DP  - 1999 Jun
TI  - FcepsilonRI-mediated antigen endocytosis turns interferon-gamma-treated mouse 
      mast cells from inefficient into potent antigen-presenting cells.
PG  - 333-40
AB  - Previous studies in our laboratory have shown that bone-marrow-derived mast cells 
      (BMMC) could present immunogenic peptides, from soluble antigens endocytosed 
      through fluid phase, only if they were subjected to a 48-hr treatment with 
      interleukin-4 (IL-4) and granulocyte-macrophage colony-stimulating factor 
      (GM-CSF). In contrast to GM-CSF, interferon-gamma (IFN-gamma) which highly 
      upregulates major histocompatibility complex (MHC) class II expression, 
      completely inhibits the generation of immunogenic peptides. We have used this 
      model to study the role of FcepsilonRI-mediated antigen internalization in the 
      regulation of the antigen-presenting function of IFN-gamma-treated mast cells. 
      Here, we report that FcepsilonRI can reverse the IFN-gamma-treated mast cells 
      from inefficient to highly efficient antigen-presenting cells. Inhibition of the 
      antigen presenting capacity by piceatannol, a protein tyrosine kinase (PTK) syk 
      inhibitor, indicates that this is an active process resulting from immunoglobulin 
      E (IgE)-antigen-FcepsilonRI engagement which involves tyrosines found in the 
      immunoreceptor tyrosine-based activation motif (ITAM) embedded in the cytoplasmic 
      tail of the FcepsilonRI beta and gamma chains. Antigen-presenting function was 
      also shown to require the activation of phosphatidyl inositol 3 (PI3) kinase, 
      downstream of PTK syk phosphorylation, since this activity was completely blocked 
      by wortmannin, a PI3 kinase inhibitor. These data suggest that signalling 
      generated by FcepsilonRI provides mast cells with IgE-mediated enhanced antigen 
      presentation to T cells and emphasize a so far unknown immunoregulatory mast-cell 
      function that might take place in inflammatory sites.
FAU - Tkaczyk, C
AU  - Tkaczyk C
AD  - Unite d'immuno-allergie, Institut Pasteur, Paris, France.
FAU - Villa, I
AU  - Villa I
FAU - Peronet, R
AU  - Peronet R
FAU - David, B
AU  - David B
FAU - Mecheri, S
AU  - Mecheri S
LA  - eng
PT  - Journal Article
PL  - England
TA  - Immunology
JT  - Immunology
JID - 0374672
RN  - 0 (Dinitrophenols)
RN  - 0 (Haptens)
RN  - 0 (Receptors, IgE)
RN  - 0 (Recombinant Proteins)
RN  - 0 (dinitrophenol-ovalbumin)
RN  - 37341-29-0 (Immunoglobulin E)
RN  - 82115-62-6 (Interferon-gamma)
RN  - 9006-59-1 (Ovalbumin)
SB  - IM
MH  - Animals
MH  - Antigen Presentation/immunology
MH  - Antigen-Presenting Cells/*immunology
MH  - Bone Marrow/immunology
MH  - Dinitrophenols/immunology
MH  - Endocytosis/*immunology
MH  - Haptens/immunology
MH  - Hydrogen-Ion Concentration
MH  - Immunoglobulin E/immunology
MH  - Interferon-gamma/*immunology
MH  - Lysosomes/immunology
MH  - Mast Cells/*immunology
MH  - Mice
MH  - Mice, Inbred DBA
MH  - Ovalbumin/immunology
MH  - Receptors, IgE/*immunology
MH  - Recombinant Proteins
PMC - PMC2326822
EDAT- 1999/08/14 10:00
MHDA- 2001/07/04 10:01
PMCR- 2000/06/01
CRDT- 1999/08/14 10:00
PHST- 1999/08/14 10:00 [pubmed]
PHST- 2001/07/04 10:01 [medline]
PHST- 1999/08/14 10:00 [entrez]
PHST- 2000/06/01 00:00 [pmc-release]
AID - imm789 [pii]
AID - 10.1046/j.1365-2567.1999.00789.x [doi]
PST - ppublish
SO  - Immunology. 1999 Jun;97(2):333-40. doi: 10.1046/j.1365-2567.1999.00789.x.