PMID- 10449448
OWN - NLM
STAT- MEDLINE
DCOM- 19990902
LR  - 20200724
IS  - 0095-1137 (Print)
IS  - 1098-660X (Electronic)
IS  - 0095-1137 (Linking)
VI  - 37
IP  - 9
DP  - 1999 Sep
TI  - Fluorescence In situ hybridization assay using peptide nucleic acid probes for 
      differentiation between tuberculous and nontuberculous mycobacterium species in 
      smears of mycobacterium cultures.
PG  - 2760-5
AB  - TB PNA FISH is a new fluorescence in situ hybridization (FISH) method using 
      peptide nucleic acid (PNA) probes for differentiation between species of the 
      Mycobacterium tuberculosis complex (MTC) and nontuberculous mycobacteria (NTM) in 
      acid-fast bacillus-positive (AFB+) cultures is described. The test is based on 
      fluorescein-labelled PNA probes that target the rRNA of MTC or NTM species 
      applied to smears of AFB+ cultures for microscopic examination. Parallel testing 
      with the two probes serves as an internal control for each sample such that a 
      valid test result is based on one positive and one negative reaction. TB PNA FISH 
      was evaluated with 30 AFB+ cultures from Denmark and 42 AFB+ cultures from 
      Thailand. The MTC-specific PNA probe showed diagnostic sensitivities of 84 and 
      97%, respectively, and a diagnostic specificity of 100% in both studies, whereas 
      the NTM-specific PNA probe showed diagnostic sensitivities of 91 and 64%, 
      respectively, and a diagnostic specificity of 100% in both studies. The low 
      sensitivity of the NTM-specific PNA probe in the Thai study was due to a 
      relatively high prevalence of Mycobacterium fortuitum, which is not identified by 
      the probe. In total, 63 (87%) of the cultures were correctly identified as MTC (n 
      = 46) or NTM (n = 17), whereas the remaining 9 were negative with both probes and 
      thus the results were inconclusive. None of the samples were incorrectly 
      identified as MTC or NTM; thus, the predictive value of a valid test result 
      obtained with TB PNA FISH was 100%.
FAU - Stender, H
AU  - Stender H
AD  - DAKO A/S, 2600 Glostrup, Denmark.
FAU - Lund, K
AU  - Lund K
FAU - Petersen, K H
AU  - Petersen KH
FAU - Rasmussen, O F
AU  - Rasmussen OF
FAU - Hongmanee, P
AU  - Hongmanee P
FAU - Miorner, H
AU  - Miorner H
FAU - Godtfredsen, S E
AU  - Godtfredsen SE
LA  - eng
PT  - Journal Article
PL  - United States
TA  - J Clin Microbiol
JT  - Journal of clinical microbiology
JID - 7505564
RN  - 0 (DNA, Ribosomal)
RN  - 0 (Nucleic Acid Probes)
RN  - 0 (Peptide Nucleic Acids)
SB  - IM
MH  - Base Sequence
MH  - DNA, Ribosomal/chemistry
MH  - Humans
MH  - *In Situ Hybridization, Fluorescence
MH  - Molecular Sequence Data
MH  - Mycobacterium/*genetics
MH  - *Nucleic Acid Probes
MH  - *Peptide Nucleic Acids
MH  - Tuberculosis/*diagnosis
PMC - PMC85371
EDAT- 1999/08/17 00:00
MHDA- 1999/08/17 00:01
PMCR- 1999/09/01
CRDT- 1999/08/17 00:00
PHST- 1999/08/17 00:00 [pubmed]
PHST- 1999/08/17 00:01 [medline]
PHST- 1999/08/17 00:00 [entrez]
PHST- 1999/09/01 00:00 [pmc-release]
AID - 0242 [pii]
AID - 10.1128/JCM.37.9.2760-2765.1999 [doi]
PST - ppublish
SO  - J Clin Microbiol. 1999 Sep;37(9):2760-5. doi: 10.1128/JCM.37.9.2760-2765.1999.