PMID- 10449898
OWN - NLM
STAT- MEDLINE
DCOM- 19991015
LR  - 20171101
IS  - 0301-0171 (Print)
IS  - 0301-0171 (Linking)
VI  - 85
IP  - 3-4
DP  - 1999
TI  - Aneuploidy in pig sperm: multicolor fluorescence in situ hybridization using 
      probes for chromosomes 1, 10, and Y.
PG  - 200-4
AB  - The objective of this research was to develop chromosome-specific probes for use 
      in evaluating aneuploidy in boar spermatozoa through the application of 
      fluorescence in situ hybridization (FISH) technology. A multicolor FISH method 
      was developed to detect aneuploidy in the sperm of boars using DNA probes 
      specific for small regions of chromosomes 1, 10, and Y. The average frequencies 
      of sperm with disomy for chromosomes 1, 10, and Y were 0.075%, 0.067%, and 
      0.094%, respectively. The incidence of disomy did not differ significantly by 
      chromosome. The average frequencies of diploidy were 0.177% for 1-1-10-10 and 
      0.022% for Y-Y-10-10. Thus, the incidence of overall diploidy (1-1-10-10) was 
      significantly higher than that of disomy for the chromosomes examined (P < 0.01 
      for disomy of the autosomes and P < 0.05 for disomy of the Y chromosome). No 
      significant age or breed effects on disomy and diploidy rates and no significant 
      interindividual variations in disomy or diploidy were found. The observed level 
      of numerical chromosome aberrations in pig sperm appear to be within the range of 
      the baseline frequencies reported so far in men.
FAU - Rubes, J
AU  - Rubes J
AD  - Veterinary Research Institute, Brno, Czech Republic. rubes@vri.cz
FAU - Vozdova, M
AU  - Vozdova M
FAU - Kubickova, S
AU  - Kubickova S
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Switzerland
TA  - Cytogenet Cell Genet
JT  - Cytogenetics and cell genetics
JID - 0367735
RN  - 0 (DNA Probes)
SB  - IM
MH  - *Aneuploidy
MH  - Animals
MH  - Cell Nucleus/ultrastructure
MH  - Chromosomes/*genetics
MH  - DNA Probes/*genetics
MH  - In Situ Hybridization, Fluorescence/*methods
MH  - Male
MH  - Sensitivity and Specificity
MH  - Spermatozoa/*ultrastructure
MH  - Swine/*genetics
EDAT- 1999/08/18 00:00
MHDA- 1999/08/18 00:01
CRDT- 1999/08/18 00:00
PHST- 1999/08/18 00:00 [pubmed]
PHST- 1999/08/18 00:01 [medline]
PHST- 1999/08/18 00:00 [entrez]
AID - 15293 [pii]
AID - 10.1159/000015293 [doi]
PST - ppublish
SO  - Cytogenet Cell Genet. 1999;85(3-4):200-4. doi: 10.1159/000015293.