PMID- 10449907 OWN - NLM STAT- MEDLINE DCOM- 19991015 LR - 20171101 IS - 0301-0171 (Print) IS - 0301-0171 (Linking) VI - 85 IP - 3-4 DP - 1999 TI - Physical assignment of the bovine MHC class IIa and class IIb genes. PG - 244-7 AB - Screening of a bovine yeast artificial chromosome (YAC) library revealed two clones which contain most of the class II genes of the major histocompatibility complex (MHC) known to date. The YACs were mapped by fluorescence in situ hybridization (FISH) and characterized for the class II genes they contain. We found that the classic class II genes BoLA- DQA, -DQB, -DRA, and -DRB3 are located at BTA 23q21 and the non-classic class II genes DYA, DIB, LMP2, LMP7, TAP2, BoLA-DOB, -DMA, -DMB, and -DNA are located at BTA 23q12-->q13. These two different mapping locations confirm and extend previous findings of a gross physical distance between classic and non-classic MHC class II genes in cattle. FAU - Hess, M AU - Hess M AD - Department of Molecular Medicine, Bernhard Nocht Institute for Tropical Medicine, Hamburg, Germany. FAU - Goldammer, T AU - Goldammer T FAU - Gelhaus, A AU - Gelhaus A FAU - Ried, K AU - Ried K FAU - Rappold, G AU - Rappold G FAU - Eggen, A AU - Eggen A FAU - Bishop, M D AU - Bishop MD FAU - Schwerin, M AU - Schwerin M FAU - Horstmann, R D AU - Horstmann RD LA - eng SI - GENBANK/AF109677 SI - GENBANK/AF110028 SI - GENBANK/AF110317 SI - GENBANK/AF110318 SI - GENBANK/AF110319 SI - GENBANK/AF110320 SI - GENBANK/AF110321 SI - GENBANK/AF110395 PT - Journal Article PT - Research Support, Non-U.S. Gov't PL - Switzerland TA - Cytogenet Cell Genet JT - Cytogenetics and cell genetics JID - 0367735 SB - IM MH - Animals MH - Cattle/*genetics MH - *Chromosome Mapping MH - Chromosomes/genetics MH - Chromosomes, Artificial, Yeast/genetics MH - Cloning, Molecular MH - Electrophoresis, Polyacrylamide Gel MH - *Genes, MHC Class II MH - Genomic Library MH - In Situ Hybridization, Fluorescence MH - Polymerase Chain Reaction MH - Sequence Analysis, DNA EDAT- 1999/08/18 00:00 MHDA- 1999/08/18 00:01 CRDT- 1999/08/18 00:00 PHST- 1999/08/18 00:00 [pubmed] PHST- 1999/08/18 00:01 [medline] PHST- 1999/08/18 00:00 [entrez] AID - 15302 [pii] AID - 10.1159/000015302 [doi] PST - ppublish SO - Cytogenet Cell Genet. 1999;85(3-4):244-7. doi: 10.1159/000015302.