PMID- 10451120
OWN - NLM
STAT- MEDLINE
DCOM- 19990910
LR  - 20071115
IS  - 0173-0835 (Print)
IS  - 0173-0835 (Linking)
VI  - 20
IP  - 10
DP  - 1999 Jul
TI  - Changes in myocardial protein expression in pacing-induced canine heart failure.
PG  - 2086-93
AB  - Canine rapid ventricular pacing produces a low output cardiomyopathic state which 
      is similar to dilated cardiomyopathy. In this study dogs were paced at 245 beats 
      per minute (bpm) for 3-4 weeks until signs of heart failure were apparent. 
      Unpaced dogs were used as controls. A previous study identified myocardial 
      protein changes in the pH region 4-7 following ventricular pacing by using 
      two-dimensional electrophoresis (2-DE) (Heinke et al., Electrophoresis 1998 19, 
      2021-2030). Many of these proteins were associated with mitochondria, energy 
      metabolism within the cardiomyocyte, the cytoskeleton and calcium cycling. The 
      present study aimed to examine the proteins migrating in the more basic region of 
      the 2-DE pattern using immobilised pH gradient 3-10 strips to separate myocardial 
      proteins. The expression of 31 proteins was altered in the paced myocardium: 21 
      were decreased and 10 increased. Following the identification of 23 of these 
      spots by either amino acid compositional analysis or peptide mass fingerprinting 
      or a combination of both, we confirm that many of the proteins whose expression 
      is altered following ventricular pacing are associated with the mitochondria and 
      energy production within the cardiomyocyte, including creatine kinase M, 
      triosephosphate isomerase, phosphoglycerate mutase, cytochrome c oxidase, 
      cytochrome b5, hydroxymethyl glutaryl CoA synthase, myoglobin, and 
      3,2-trans-enoyl-CoA transferase. Additionally, the cytoskeletal protein actin was 
      increased in the paced hearts. These results strongly support the notion that 
      energy production is impaired and mitochondrial dysfunction is involved in the 
      development of heart failure in the paced dog.
FAU - Heinke, M Y
AU  - Heinke MY
AD  - Institute for Biomedical Research, The University of Sydney, NSW, Australia. 
      monique@anatomy.usyd.edu.au
FAU - Wheeler, C H
AU  - Wheeler CH
FAU - Yan, J X
AU  - Yan JX
FAU - Amin, V
AU  - Amin V
FAU - Chang, D
AU  - Chang D
FAU - Einstein, R
AU  - Einstein R
FAU - Dunn, M J
AU  - Dunn MJ
FAU - dos Remedios, C G
AU  - dos Remedios CG
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Germany
TA  - Electrophoresis
JT  - Electrophoresis
JID - 8204476
RN  - 0 (Proteins)
SB  - IM
MH  - Animals
MH  - Blotting, Western
MH  - Cardiac Pacing, Artificial
MH  - Cardiomyopathy, Dilated/etiology/metabolism
MH  - Disease Models, Animal
MH  - Dogs
MH  - Electrophoresis, Gel, Two-Dimensional
MH  - Energy Metabolism
MH  - Female
MH  - Heart Failure/etiology/*metabolism
MH  - Heart Rate
MH  - Heart Ventricles/physiopathology
MH  - Hydrogen-Ion Concentration
MH  - Male
MH  - Mitochondria, Heart/metabolism
MH  - Myocardium/chemistry/*metabolism
MH  - Proteins/analysis/*metabolism
MH  - Silver Staining
EDAT- 1999/08/18 10:00
MHDA- 2000/08/12 11:00
CRDT- 1999/08/18 10:00
PHST- 1999/08/18 10:00 [pubmed]
PHST- 2000/08/12 11:00 [medline]
PHST- 1999/08/18 10:00 [entrez]
AID - 10.1002/(SICI)1522-2683(19990701)20:10<2086::AID-ELPS2086>3.0.CO;2-4 [pii]
AID - 10.1002/(SICI)1522-2683(19990701)20:10<2086::AID-ELPS2086>3.0.CO;2-4 [doi]
PST - ppublish
SO  - Electrophoresis. 1999 Jul;20(10):2086-93. doi: 
      10.1002/(SICI)1522-2683(19990701)20:10<2086::AID-ELPS2086>3.0.CO;2-4.