PMID- 10451701
OWN - NLM
STAT- MEDLINE
DCOM- 19990902
LR  - 20061115
IS  - 1045-2257 (Print)
IS  - 1045-2257 (Linking)
VI  - 24
IP  - 3
DP  - 1999 Mar
TI  - Characterization of the human myeloid leukemia-derived cell line GF-D8 by 
      multiplex fluorescence in situ hybridization, subtelomeric probes, and 
      comparative genomic hybridization.
PG  - 213-21
AB  - The human myeloid leukemia cell line GF-D8 was established from the peripheral 
      blood blasts of a patient with acute myeloid leukemia FAB subtype MI (AML-MI). 
      The karyotype, which has not changed significantly over several years of culture, 
      was described initially as 
      44,XY,-5,del(7q),inv(7q),add(8q),add(11q),del(12p),-15,-17,+mar. With the advent 
      of multicolor fluorescence in situ hybridization (FISH) techniques, the prospect 
      of accurately characterizing this complex karyotype became feasible. In the 
      present study, we applied 24-color whole-chromosome painting and analyzed the 
      results using a filter-based detection system and proprietary software for 
      multiplex FISH (M-FISH). This resulted in the refinement of the karyotype and the 
      identification of hitherto unsuspected chromosome rearrangements. M-FISH 
      identified the origin of the add(8q) and add(11q) as well as the small marker 
      chromosome. Both the del(7q) and del(12p) were redefined as unbalanced 
      translocations and an apparently normal chromosome 11 was shown to be t(11;17). 
      Importantly, the del(12p) was shown to be a der(12)t(7;12). Single-color 
      whole-chromosome painting studies confirmed these findings, but also identified a 
      cryptic t(Y;12) not seen in the original M-FISH analysis. We then carried out a 
      FISH screening assay using a complete set of chromosome-specific subtelomeric 
      probes. This allowed the identification of p and q subtelomeric regions involved 
      in the translocations and indicated amplification of the 8q subtelomeric region. 
      Comparative genomic hybridization (CGH) revealed a highly unbalanced karyotype, 
      as deletions accompanied the majority of translocations, and identified the 
      regions of amplification as 8q22.3-qter and 11q21-qter. Finally, conventional 
      FISH with centromeric and unique sequence probes was necessary to elucidate all 
      of the rearrangements.
FAU - Tosi, S
AU  - Tosi S
AD  - Institute of Molecular Medicine, Headington, Oxford, England.
FAU - Giudici, G
AU  - Giudici G
FAU - Rambaldi, A
AU  - Rambaldi A
FAU - Scherer, S W
AU  - Scherer SW
FAU - Bray-Ward, P
AU  - Bray-Ward P
FAU - Dirscherl, L
AU  - Dirscherl L
FAU - Biondi, A
AU  - Biondi A
FAU - Kearney, L
AU  - Kearney L
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Genes Chromosomes Cancer
JT  - Genes, chromosomes & cancer
JID - 9007329
RN  - 0 (DNA Probes)
SB  - IM
MH  - Aged
MH  - Aged, 80 and over
MH  - Chromosome Mapping
MH  - Chromosome Painting
MH  - DNA Probes/*genetics
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Karyotyping
MH  - Leukemia, Myeloid/*genetics/*metabolism
MH  - Male
MH  - Mutation
MH  - Nucleic Acid Hybridization
MH  - Repetitive Sequences, Nucleic Acid/genetics
MH  - Telomere/*genetics
MH  - Tumor Cells, Cultured/*chemistry
EDAT- 1999/08/19 10:00
MHDA- 2000/06/20 09:00
CRDT- 1999/08/19 10:00
PHST- 1999/08/19 10:00 [pubmed]
PHST- 2000/06/20 09:00 [medline]
PHST- 1999/08/19 10:00 [entrez]
AID - 10.1002/(SICI)1098-2264(199903)24:3<213::AID-GCC6>3.0.CO;2-B [pii]
PST - ppublish
SO  - Genes Chromosomes Cancer. 1999 Mar;24(3):213-21.