PMID- 10454345
OWN - NLM
STAT- MEDLINE
DCOM- 19991019
LR  - 20071114
IS  - 1079-9907 (Print)
IS  - 1079-9907 (Linking)
VI  - 19
IP  - 7
DP  - 1999 Jul
TI  - Production of influenza-stimulated tumor necrosis factor-alpha by monocytes 
      following acute influenza infection in humans.
PG  - 751-5
AB  - Tumor necrosis factor-alpha (TNF-alpha) is often measured in the serum or plasma 
      of patients with severe infections, and marked elevation correlates with poor 
      outcome. The relationship of TNF-alpha to protection from disease is frequently 
      not observed because prospective studies of infectious agents are difficult to 
      perform. We took advantage of a human antiviral influenza challenge study to 
      correlate TNF-alpha production with seroconversion and symptom development. 
      TNF-alpha production was measured by ELISA in the plasma compartment or was 
      measured by intracellular production at the single cell level in the monocyte 
      gated population. Monocyte TNF-alpha was associated with asymptomatic 
      seroconversion, whereas there was no change in the plasma at the times measured. 
      Measurement of TNF-alpha at the single cell level by flow cytometry may allow for 
      better differentiation of the protective role of this cytokine in future studies.
FAU - Kallas, E G
AU  - Kallas EG
AD  - Infectious Diseases Unit, University of Rochester, NY 14642, USA.
FAU - Reynolds, K
AU  - Reynolds K
FAU - Andrews, J
AU  - Andrews J
FAU - Treanor, J J
AU  - Treanor JJ
FAU - Evans, T G
AU  - Evans TG
LA  - eng
GR  - N0I-AI-45208/AI/NIAID NIH HHS/United States
GR  - N0I-AI-45248/AI/NIAID NIH HHS/United States
PT  - Clinical Trial
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - J Interferon Cytokine Res
JT  - Journal of interferon & cytokine research : the official journal of the 
      International Society for Interferon and Cytokine Research
JID - 9507088
RN  - 0 (Tumor Necrosis Factor-alpha)
SB  - IM
MH  - Adolescent
MH  - Adult
MH  - Flow Cytometry
MH  - Humans
MH  - Influenza A virus/*physiology
MH  - Influenza, Human/*blood
MH  - Leukocytes, Mononuclear/metabolism/*virology
MH  - Tumor Necrosis Factor-alpha/*biosynthesis
EDAT- 1999/08/24 00:00
MHDA- 1999/08/24 00:01
CRDT- 1999/08/24 00:00
PHST- 1999/08/24 00:00 [pubmed]
PHST- 1999/08/24 00:01 [medline]
PHST- 1999/08/24 00:00 [entrez]
AID - 10.1089/107999099313596 [doi]
PST - ppublish
SO  - J Interferon Cytokine Res. 1999 Jul;19(7):751-5. doi: 10.1089/107999099313596.