PMID- 10454579
OWN - NLM
STAT- MEDLINE
DCOM- 19990910
LR  - 20240407
IS  - 0270-7306 (Print)
IS  - 1098-5549 (Electronic)
IS  - 0270-7306 (Linking)
VI  - 19
IP  - 9
DP  - 1999 Sep
TI  - Activating signal cointegrator 1, a novel transcription coactivator of nuclear 
      receptors, and its cytosolic localization under conditions of serum deprivation.
PG  - 6323-32
AB  - Activating signal cointegrator 1 (ASC-1) harbors an autonomous transactivation 
      domain that contains a putative zinc finger motif which provides binding sites 
      for basal transcription factors TBP and TFIIA, transcription integrators steroid 
      receptor coactivator 1 (SRC-1) and CBP-p300, and nuclear receptors, as 
      demonstrated by the glutathione S-transferase pull-down assays and the yeast 
      two-hybrid tests. The ASC-1 binding sites involve the hinge domain but not the 
      C-terminal AF2 core domain of nuclear receptors. Nonetheless, ASC-1 appears to 
      require the AF2-dependent factors to function (i.e., CBP-p300 and SRC-1), as 
      suggested by the ability of ASC-1 to coactivate nuclear receptors, either alone 
      or in cooperation with SRC-1 and p300, as well as its inability to coactivate a 
      mutant receptor lacking the AF2 core domain. By using indirect 
      immunofluorescence, we further show that ASC-1, a nuclear protein, is localized 
      to the cytoplasm under conditions of serum deprivation but is retained in the 
      nucleus when it is serum starved in the presence of ligand or coexpressed CBP or 
      SRC-1. These results suggest that ASC-1 is a novel coactivator molecule of 
      nuclear receptors which functions in conjunction with CBP-p300 and SRC-1 and may 
      play an important role in establishing distinct coactivator complexes under 
      different cellular conditions.
FAU - Kim, H J
AU  - Kim HJ
AD  - Center for Ligand and Transcription, Chonnam National University, Kwangju 
      500-757, Korea.
FAU - Yi, J Y
AU  - Yi JY
FAU - Sung, H S
AU  - Sung HS
FAU - Moore, D D
AU  - Moore DD
FAU - Jhun, B H
AU  - Jhun BH
FAU - Lee, Y C
AU  - Lee YC
FAU - Lee, J W
AU  - Lee JW
LA  - eng
SI  - GENBANK/AF168418
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Mol Cell Biol
JT  - Molecular and cellular biology
JID - 8109087
RN  - 0 (Culture Media, Serum-Free)
RN  - 0 (DNA, Complementary)
RN  - 0 (Nuclear Proteins)
RN  - 0 (Receptors, Cytoplasmic and Nuclear)
RN  - 0 (Receptors, Retinoic Acid)
RN  - 0 (Receptors, Steroid)
RN  - 0 (Retinoid X Receptors)
RN  - 0 (Trans-Activators)
RN  - 0 (Transcription Factors)
RN  - EC 2.3.1.48 (Histone Acetyltransferases)
RN  - EC 2.3.1.48 (NCOA1 protein, human)
RN  - EC 2.3.1.48 (Nuclear Receptor Coactivator 1)
SB  - IM
MH  - Amino Acid Sequence
MH  - Animals
MH  - Binding Sites
MH  - Cell Line
MH  - Cell Nucleus/metabolism
MH  - Culture Media, Serum-Free
MH  - Cytosol/metabolism
MH  - DNA, Complementary/genetics/isolation & purification
MH  - HeLa Cells
MH  - Histone Acetyltransferases
MH  - Humans
MH  - Models, Biological
MH  - Molecular Sequence Data
MH  - Nuclear Proteins/metabolism
MH  - Nuclear Receptor Coactivator 1
MH  - Receptors, Cytoplasmic and Nuclear/*metabolism
MH  - Receptors, Retinoic Acid/metabolism
MH  - Receptors, Steroid/metabolism
MH  - Retinoid X Receptors
MH  - Sequence Homology, Amino Acid
MH  - Signal Transduction
MH  - Trans-Activators/chemistry/genetics/*metabolism
MH  - Transcription Factors/metabolism
MH  - Zinc Fingers
PMC - PMC84603
EDAT- 1999/08/24 00:00
MHDA- 1999/08/24 00:01
PMCR- 1999/09/01
CRDT- 1999/08/24 00:00
PHST- 1999/08/24 00:00 [pubmed]
PHST- 1999/08/24 00:01 [medline]
PHST- 1999/08/24 00:00 [entrez]
PHST- 1999/09/01 00:00 [pmc-release]
AID - 1659 [pii]
AID - 10.1128/MCB.19.9.6323 [doi]
PST - ppublish
SO  - Mol Cell Biol. 1999 Sep;19(9):6323-32. doi: 10.1128/MCB.19.9.6323.