PMID- 10455895
OWN - NLM
STAT- MEDLINE
DCOM- 19990902
LR  - 20171207
IS  - 0039-6060 (Print)
IS  - 0039-6060 (Linking)
VI  - 126
IP  - 2
DP  - 1999 Aug
TI  - Trauma-hemorrhage delays wound healing potentially by increasing pro-inflammatory 
      cytokines at the wound site.
PG  - 279-85
AB  - BACKGROUND: Studies indicate impaired wound healing after trauma. The underlying 
      mechanism remains unknown. METHODS: Mice were subjected to midline laparotomy, 
      and polyvinyl alcohol sponges were implanted subcutaneously before hemorrhage (35 
      +/- 5 mmHg for 90 minutes, resuscitated) or sham operation. Wound exudate cells 
      from the sponges were harvested on the first, third, and fifth postoperative day 
      and cultured for 24 hours. Interleukin (IL)-1 beta, IL-6, monocyte chemotactic 
      protein-1 (MCP-1), macrophage inflammatory protein-2 (MIP-2), and transforming 
      growth factor (TGF)-beta were determined in the supernatants. IL-1 beta and IL-6 
      were measured in the wound fluid. RESULTS: Hemorrhage decreased collagen 
      deposition in the wound. TGF-beta release was significantly decreased on the 
      first and third postoperative days after hemorrhage, whereas IL-1 beta and IL-6 
      release was increased at 3 and 5 days after hemorrhage. Similarly, IL-1 beta and 
      IL-6 in the wound fluid were significantly increased at 3 days after hemorrhage. 
      CONCLUSIONS: Because increased levels of pro-inflammatory cytokines and decreased 
      amounts of TGF-beta have been reported to impair the process of wound healing, 
      the increased release of IL-1 beta and IL-6 and the decreased release of TGF-beta 
      after hemorrhage might contribute to the decreased collagen production in those 
      animals. Thus, attempts to locally change the ratio of those cytokines in trauma 
      victims might be useful for improving wound healing in those patients.
FAU - Angele, M K
AU  - Angele MK
AD  - Center for Surgical Research, Brown University School of Medicine, Providence, 
      RI, USA.
FAU - Knoferl, M W
AU  - Knoferl MW
FAU - Ayala, A
AU  - Ayala A
FAU - Albina, J E
AU  - Albina JE
FAU - Cioffi, W G
AU  - Cioffi WG
FAU - Bland, K I
AU  - Bland KI
FAU - Chaudry, I H
AU  - Chaudry IH
LA  - eng
GR  - R01 GM 37127/GM/NIGMS NIH HHS/United States
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Surgery
JT  - Surgery
JID - 0417347
RN  - 0 (Chemokines)
RN  - 0 (Cytokines)
SB  - IM
MH  - Animals
MH  - Chemokines/biosynthesis
MH  - Cytokines/*biosynthesis
MH  - Hemorrhage/*metabolism
MH  - Macrophages/physiology
MH  - Male
MH  - Mice
MH  - Mice, Inbred C3H
MH  - Neutrophils/physiology
MH  - *Wound Healing
MH  - Wounds and Injuries/*metabolism
EDAT- 1999/08/24 00:00
MHDA- 1999/08/24 00:01
CRDT- 1999/08/24 00:00
PHST- 1999/08/24 00:00 [pubmed]
PHST- 1999/08/24 00:01 [medline]
PHST- 1999/08/24 00:00 [entrez]
AID - S0039-6060(99)70166-2 [pii]
PST - ppublish
SO  - Surgery. 1999 Aug;126(2):279-85.