PMID- 10456846
OWN - NLM
STAT- MEDLINE
DCOM- 19990920
LR  - 20190513
IS  - 0006-3363 (Print)
IS  - 0006-3363 (Linking)
VI  - 61
IP  - 3
DP  - 1999 Sep
TI  - Glyceraldehyde-3-phosphate dehydrogenase expression during apoptosis and 
      proliferation of rat ventral prostate.
PG  - 687-91
AB  - Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) is a multifunctional enzyme 
      known to play a critical role in neuronal apoptosis. We undertook the current 
      studies to determine whether GAPDH also plays a role in prostate epithelial cell 
      apoptosis in response to androgen deprivation. To do so, we analyzed GAPDH 
      staining by immunohistochemistry during castration-induced involution and 
      androgen-induced regeneration of rat ventral prostate. We found that GAPDH was 
      undetectable in secretory epithelial cells at baseline and that staining did not 
      increase in the epithelium during the period of peak apoptosis from 1 to 3 days 
      after castration. However, GAPDH levels did increase within nuclei of some basal 
      epithelial cells 5 days after castration and within the cytoplasm of all 
      secretory epithelial cells 7 days after castration. GAPDH was also abundant 
      within the cytoplasm of secretory epithelial cells during the period of maximal 
      cell proliferation from 2 to 3 days after androgen replacement and was clearly 
      apparent within nuclei of some epithelial cells 4 days after androgen 
      replacement. Our studies suggest that GAPDH plays multiple roles during prostate 
      epithelial cell apoptosis and proliferation.
FAU - Epner, D E
AU  - Epner DE
AD  - Department of Medicine, Baylor College of Medicine and VA Medical Center, 
      Houston, Texas 77030, USA. depner@bcm.tmc.edu
FAU - Sawa, A
AU  - Sawa A
FAU - Isaacs, J T
AU  - Isaacs JT
LA  - eng
GR  - 1 R29CA78355-01/CA/NCI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, Non-P.H.S.
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Biol Reprod
JT  - Biology of reproduction
JID - 0207224
RN  - 3XMK78S47O (Testosterone)
RN  - EC 1.2.1.- (Glyceraldehyde-3-Phosphate Dehydrogenases)
SB  - IM
MH  - Animals
MH  - *Apoptosis
MH  - *Cell Division
MH  - Cell Nucleus/enzymology
MH  - Cytoplasm/enzymology
MH  - Epithelial Cells/enzymology/ultrastructure
MH  - Glyceraldehyde-3-Phosphate Dehydrogenases/analysis/*metabolism
MH  - Immunohistochemistry
MH  - Male
MH  - Orchiectomy
MH  - Prostate/*cytology/*enzymology
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Testosterone/pharmacology
EDAT- 1999/08/24 00:00
MHDA- 1999/08/24 00:01
CRDT- 1999/08/24 00:00
PHST- 1999/08/24 00:00 [pubmed]
PHST- 1999/08/24 00:01 [medline]
PHST- 1999/08/24 00:00 [entrez]
AID - 10.1095/biolreprod61.3.687 [doi]
PST - ppublish
SO  - Biol Reprod. 1999 Sep;61(3):687-91. doi: 10.1095/biolreprod61.3.687.