PMID- 10457216
OWN - NLM
STAT- MEDLINE
DCOM- 19991004
LR  - 20231213
IS  - 0019-2805 (Print)
IS  - 1365-2567 (Electronic)
IS  - 0019-2805 (Linking)
VI  - 97
IP  - 4
DP  - 1999 Aug
TI  - Enhanced antigen-presenting activity and tumour necrosis factor-alpha-independent 
      activation of dendritic cells following treatment with Mycobacterium bovis 
      bacillus Calmette-Guerin.
PG  - 626-33
AB  - Dendritic cells (DCs) are most potent among the antigen-presenting cells and are 
      believed to be crucial for the initiation of a primary T-cell response to foreign 
      antigens. Mycobacterial infection within macrophages is controlled by 
      cell-mediated immunity. To elucidate the stimulation of immune response by 
      Mycobacterium bovis bacillus Calmette-Guerin (BCG), we purified DCs from 
      precursor cells in human peripheral blood mononuclear cells (PBMC) by culturing 
      them with granulocyte-macrophage colony-stimulating factor (GM-CSF) and 
      interleukin-4 (IL-4) and characterized their surface antigen expression. The 
      interaction of cultured DCs with BCG resulted in increased surface expression of 
      several DC-related marker antigens. BCG also induced reduction of endocytosis, 
      enhancement of CD83 expression as well as B7 costimulatory molecules and IL-12 
      production, suggesting that BCG treatment directly induces DCs to mature. 
      BCG-treated DCs were much more potent antigen-presenting cells in allogeneic 
      immune response than untreated DCs. Moreover, while the neutralization of tumour 
      necrosis factor-alpha (TNF-alpha) significantly blocked the DC maturation induced 
      by lipopolysaccharide (LPS), it could not inhibit the induction of DC maturation 
      by the BCG treatment, indicating that TNF-alpha production plays a minor role in 
      the BCG-induced DC maturation. However, the neutralization of TNF-alpha resulted 
      in decreased IL-12 production by activated DCs. These results suggest that 
      infection with BCG might evoke direct activation and maturation of DC and the 
      general immune stimulant effect of BCG might be related with the activation of 
      DCs.
FAU - Kim, K D
AU  - Kim KD
AD  - Korea Research Institute of Bioscience and Biotechnology, Taejon, South Korea.
FAU - Lee, H G
AU  - Lee HG
FAU - Kim, J K
AU  - Kim JK
FAU - Park, S N
AU  - Park SN
FAU - Choe, I S
AU  - Choe IS
FAU - Choe, Y K
AU  - Choe YK
FAU - Kim, S J
AU  - Kim SJ
FAU - Lee, E
AU  - Lee E
FAU - Lim, J S
AU  - Lim JS
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Immunology
JT  - Immunology
JID - 0374672
RN  - 0 (Antigens, CD)
RN  - 0 (Immunoglobulins)
RN  - 0 (Lipopolysaccharides)
RN  - 0 (Membrane Glycoproteins)
RN  - 0 (Tumor Necrosis Factor-alpha)
RN  - 187348-17-0 (Interleukin-12)
SB  - IM
MH  - Antigen Presentation/*immunology
MH  - Antigens, CD/metabolism
MH  - Cell Culture Techniques
MH  - Cell Differentiation
MH  - Dendritic Cells/*immunology
MH  - Humans
MH  - Immunoglobulins/metabolism
MH  - Interleukin-12/biosynthesis
MH  - Leukocytes, Mononuclear/immunology
MH  - Lipopolysaccharides/immunology
MH  - Lymphocyte Activation/immunology
MH  - Membrane Glycoproteins/metabolism
MH  - Mycobacterium bovis/*immunology
MH  - T-Lymphocytes/immunology
MH  - Tumor Necrosis Factor-alpha/*immunology
MH  - CD83 Antigen
PMC - PMC2326884
EDAT- 1999/08/24 00:00
MHDA- 1999/08/24 00:01
PMCR- 2000/08/01
CRDT- 1999/08/24 00:00
PHST- 1999/08/24 00:00 [pubmed]
PHST- 1999/08/24 00:01 [medline]
PHST- 1999/08/24 00:00 [entrez]
PHST- 2000/08/01 00:00 [pmc-release]
AID - imm818 [pii]
AID - 10.1046/j.1365-2567.1999.00818.x [doi]
PST - ppublish
SO  - Immunology. 1999 Aug;97(4):626-33. doi: 10.1046/j.1365-2567.1999.00818.x.