PMID- 10458479
OWN - NLM
STAT- MEDLINE
DCOM- 19990921
LR  - 20190727
IS  - 0040-8727 (Print)
IS  - 0040-8727 (Linking)
VI  - 187
IP  - 3
DP  - 1999 Mar
TI  - Administration of nerve growth factor, brain-derived neurotrophic factor and 
      insulin-like growth factor-II protects phosphate-activated glutaminase in the 
      ischemic and reperfused rat retinas.
PG  - 227-36
AB  - Phosphate-activated glutaminase (PAG) activity decreases markedly in the early 
      period of ischemia. The decrease of the enzyme activity is reversible if the 
      ischemic period is relatively short, but it becomes irreversible after 90 minutes 
      of ischemia. The deterioration is a functional damage of the retinas caused by 
      ischemia. We studied effects of growth factors and neurotrophic factors on 
      protection of PAG in the ischemic and reperfused rat retinas. Before ischemia, 1 
      microl of growth factors or neurotrophic factors (0.1 microg/microl for 
      insulin-like growth factor-I [IGF-I], insulin-like growth factor-II [IGF-II], 
      brain-derived neurotrophic factor [BDNF], nerve growth factor [NGF]; 1 
      microg/microl for basic fibroblast growth factor [bFGF]) were injected into the 
      vitreous cavity of the left eyes of anesthetized Sprague Dawley rats. As a 
      control, phosphate buffered saline was injected to the right eyes. To induce 
      ischemia, we clamped left eyes for 90 minutes after bulbar conjunctival incision 
      all around limbus. The rat retinas were homogenized with distilled water 1 day 
      after reperfusion and used for PAG assay. Retinal ammonia concentration was also 
      determined as a ischemic marker. About 80% decrease of retinal PAG activity and 
      50% increase of retinal ammonia concentration were observed after 90 minutes of 
      ischemia and 1 day of reperfusion as compared with unoperated normal eyes. 
      IGF-II, BDNF and NGF had protective effects on the retinal PAG activity, whereas 
      IGF-I, bFGF, stable bFGF were less effective. In addition, IGF-II and BDNF 
      suppressed elevation of retinal ammonia concentration. BDNF, NGF and IGF-II have 
      marked effect on the protection of PAG activity in the ischemic and reperfused 
      rat retinas, whereas bFGF, which is very effective for the protection of ischemic 
      cell death, shows moderate effect.
FAU - Tomita, H
AU  - Tomita H
AD  - Department of Ophthalmology, Tohoku University School of Medicine, Sendai, Japan. 
      h-tomita@oph.med.tohoku.ac.jp
FAU - Ishiguro, S
AU  - Ishiguro S
FAU - Abe, T
AU  - Abe T
FAU - Tamai, M
AU  - Tamai M
LA  - eng
PT  - Journal Article
PL  - Japan
TA  - Tohoku J Exp Med
JT  - The Tohoku journal of experimental medicine
JID - 0417355
RN  - 0 (Brain-Derived Neurotrophic Factor)
RN  - 0 (Nerve Growth Factors)
RN  - 67763-97-7 (Insulin-Like Growth Factor II)
RN  - EC 3.5.1.2 (Glutaminase)
SB  - IM
MH  - Animals
MH  - Brain-Derived Neurotrophic Factor/*administration & dosage
MH  - Enzyme Activation/drug effects
MH  - Glutaminase/*metabolism
MH  - Insulin-Like Growth Factor II/*administration & dosage
MH  - Nerve Growth Factors/*administration & dosage
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Reperfusion Injury/*metabolism
MH  - Retina/metabolism/*pathology
MH  - Retinal Vessels/metabolism/pathology
EDAT- 1999/08/24 00:00
MHDA- 1999/08/24 00:01
CRDT- 1999/08/24 00:00
PHST- 1999/08/24 00:00 [pubmed]
PHST- 1999/08/24 00:01 [medline]
PHST- 1999/08/24 00:00 [entrez]
AID - 10.1620/tjem.187.227 [doi]
PST - ppublish
SO  - Tohoku J Exp Med. 1999 Mar;187(3):227-36. doi: 10.1620/tjem.187.227.