PMID- 10458897
OWN - NLM
STAT- MEDLINE
DCOM- 19991021
LR  - 20141120
IS  - 0011-2240 (Print)
IS  - 0011-2240 (Linking)
VI  - 39
IP  - 1
DP  - 1999 Aug
TI  - Platelet cryopreservation using a reduced dimethyl sulfoxide concentration and 
      second-messenger effectors as cryopreserving solution.
PG  - 1-12
AB  - Cryopreservation of platelets is of great interest since it could extend to years 
      the shelf life of therapeutic platelet concentrates (PCs) and facilitate 
      stockpiling and inventory control in blood banking. We have compared the 
      cryopreservation of PCs by the standard method using 6% Me(2)SO as cryoprotectant 
      with the method of freezing employing low concentrations of Me(2)SO (2%) plus 
      ThromboSol, a mixture of second-messenger effectors that protect platelets from 
      cold damage. PC pools were treated either with 6% Me(2)SO or with ThromboSol and 
      2% Me(2)SO and then placed directly in a -80 degrees C freezer or in the vapor 
      phase of a liquid nitrogen freezer (-120 degrees C). After storage for 1 week or 
      for 3 months, samples were removed, thawed, and analyzed. Measurements included 
      cell recovery, biochemical parameters, membrane glycoproteins (GPs), platelet 
      aggregation, and binding of radiolabeled von Willebrand factor (vWF) and 
      fibrinogen. PCs cryopreserved with ThromboSol and 2% Me(2)SO displayed a platelet 
      recovery (90%) equivalent to those frozen with 6% Me(2)SO. Following either 
      cryopreservation procedure, platelets showed increased surface expression of 
      P-selectin and moderate loss of GP Ibalpha in comparison to fresh platelets. The 
      aggregatory response to ristocetin and the binding of vWF were similar in 
      platelets frozen by either procedure. Finally, both methods promoted comparable 
      impairment of the reactivity of platelets to thrombin, aggregation and binding of 
      fibrinogen and vWF, compared to that of fresh platelets. In summary, 
      cryopreservation of PCs using reduced Me(2)SO concentration and ThromboSol yields 
      platelets with in vitro functional characteristics equivalent to those of cells 
      frozen with the conventional method using 6% Me(2)SO.
CI  - Copyright 1999 Academic Press.
FAU - Lozano, M L
AU  - Lozano ML
AD  - Unit of Hematology, Hemotherapy and Clinical Oncology, School of Medicine, Centro 
      Regional de Hemodonacion, Murcia, 30003, Spain.
FAU - Rivera, J
AU  - Rivera J
FAU - Corral, J
AU  - Corral J
FAU - Gonzalez-Conejero, R
AU  - Gonzalez-Conejero R
FAU - Vicente, V
AU  - Vicente V
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Netherlands
TA  - Cryobiology
JT  - Cryobiology
JID - 0006252
RN  - 0 (Cryoprotective Agents)
RN  - 0 (Platelet Membrane Glycoproteins)
RN  - 0 (Solutions)
RN  - 0 (von Willebrand Factor)
RN  - 1404-55-3 (Ristocetin)
RN  - 9001-32-5 (Fibrinogen)
RN  - YOW8V9698H (Dimethyl Sulfoxide)
SB  - IM
MH  - *Blood Platelets/drug effects/metabolism
MH  - Blood Preservation/*methods
MH  - Cryopreservation/*methods
MH  - Cryoprotective Agents
MH  - Dimethyl Sulfoxide
MH  - Evaluation Studies as Topic
MH  - Fibrinogen/metabolism
MH  - Humans
MH  - In Vitro Techniques
MH  - Platelet Aggregation/drug effects
MH  - Platelet Count
MH  - Platelet Membrane Glycoproteins/metabolism
MH  - Ristocetin/pharmacology
MH  - Second Messenger Systems
MH  - Solutions
MH  - von Willebrand Factor/metabolism
EDAT- 1999/08/25 00:00
MHDA- 1999/08/25 00:01
CRDT- 1999/08/25 00:00
PHST- 1999/08/25 00:00 [pubmed]
PHST- 1999/08/25 00:01 [medline]
PHST- 1999/08/25 00:00 [entrez]
AID - S0011-2240(99)92184-3 [pii]
AID - 10.1006/cryo.1999.2184 [doi]
PST - ppublish
SO  - Cryobiology. 1999 Aug;39(1):1-12. doi: 10.1006/cryo.1999.2184.