PMID- 10459340
OWN - NLM
STAT- MEDLINE
DCOM- 19990910
LR  - 20190816
IS  - 0165-4608 (Print)
IS  - 0165-4608 (Linking)
VI  - 113
IP  - 1
DP  - 1999 Aug
TI  - AgNOR of human interphase cells in relation to acrocentric chromosomes.
PG  - 14-8
AB  - Using simultaneous detection of fluorescence in situ hybridization (FISH) to 
      acrocentric chromosome centromeres and argyrophilic nucleolar organizer regions 
      (AgNOR), we investigated the number of AgNOR and involvement pattern of 
      acrocentric chromosomes in the nucleoli in various types of human interphase 
      cells. The number of AgNOR of normal gastric mucosal epithelial cells was 2.27 
      +/- 1.18 and was higher than that of lymphocytes (1.08 +/- 0.28) and lower than 
      that of gastric cancer (7.76 +/- 3.21). The number of acrocentric chromosome 
      centromere signals of normal gastric mucosal epithelial cells was higher than 
      that of normal leukocytes (P < 0.000), and lower than that of gastric cancer (P < 
      0.000). The acrocentric chromosome centromere signals in the lymphocytes and 
      neutrophils were only half of that expected for diploid cells, perhaps related to 
      acrocentric chromosome association. The proportion of acrocentric chromosomes 
      attached to AgNOR in gastric cancer (0.88 +/- 0.22) was significantly higher than 
      that of normal gastric mucosal epithelial cells (0.72 +/- 0.35, P = 0.007). In 
      conclusion, acrocentric chromosome association appears to be present in 
      circulating leukocytes even in interphase. The number of AgNORs and proportion of 
      acrocentric chromosomes involved in AgNORs in human interphase cells may vary 
      according to cell types. This could play a significant role in rDNA transcription 
      and determination of cell phenotype, including malignant change.
FAU - Lee, W
AU  - Lee W
AD  - Department of Pediatrics, Catholic University Medical College, Seoul, Korea.
FAU - Kim, Y
AU  - Kim Y
FAU - Lee, K Y
AU  - Lee KY
FAU - Kang, C S
AU  - Kang CS
FAU - Lee, W
AU  - Lee W
FAU - Lee, K S
AU  - Lee KS
FAU - Shim, S I
AU  - Shim SI
FAU - Han, K
AU  - Han K
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Cancer Genet Cytogenet
JT  - Cancer genetics and cytogenetics
JID - 7909240
SB  - IM
MH  - Cell Division/physiology
MH  - Cell Nucleolus/ultrastructure
MH  - Cells, Cultured
MH  - *Centromere
MH  - Chromosomes, Human, Pair 14
MH  - Chromosomes, Human, Pair 15
MH  - Chromosomes, Human, Pair 22
MH  - Epithelial Cells/ultrastructure
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - *Interphase
MH  - Leukocytes/ultrastructure
MH  - Nucleolus Organizer Region/*genetics
MH  - Silver Staining
MH  - Stomach Neoplasms/genetics
MH  - Tumor Cells, Cultured
EDAT- 1999/08/25 00:00
MHDA- 1999/08/25 00:01
CRDT- 1999/08/25 00:00
PHST- 1999/08/25 00:00 [pubmed]
PHST- 1999/08/25 00:01 [medline]
PHST- 1999/08/25 00:00 [entrez]
AID - S0165460898002817 [pii]
AID - 10.1016/s0165-4608(98)00281-7 [doi]
PST - ppublish
SO  - Cancer Genet Cytogenet. 1999 Aug;113(1):14-8. doi: 10.1016/s0165-4608(98)00281-7.