PMID- 10459341
OWN - NLM
STAT- MEDLINE
DCOM- 19990910
LR  - 20190816
IS  - 0165-4608 (Print)
IS  - 0165-4608 (Linking)
VI  - 113
IP  - 1
DP  - 1999 Aug
TI  - Is a duplication of 14q32 a new recurrent chromosomal alteration in B-cell 
      non-Hodgkin lymphoma?
PG  - 19-24
AB  - Identification of clonal chromosomal abnormalities involving 14q32 and its 
      association with specific histological subtypes of non-Hodgkin lymphoma (NHL) has 
      provided substantial insight to the genetic events leading to the disease. 
      However, in some cases with inferior morphology of tumor cell chromosomes, the 
      additional segment on chromosome 14 remains unidentified by cytogenetic banding 
      techniques alone. To elucidate the origin of the additional chromosomal segment 
      and to correlate the newly determined alterations with histology, metaphases from 
      15 NHL patients with add(14)(q32) were examined using fluorescence in situ 
      hybridization (FISH) techniques after cytogenetic analysis had been performed. We 
      found the duplication of 14q involving the q32 region in 6 cases with a dup(14) 
      (q32) in 4 cases and a dup(14)(q24q32) in 2 cases. In 8 cases, FISH unveiled 
      known NHL associated translocations; a t(14;18)(q32;q21) in 4 cases, a 
      t(11;14)(q13;q32) in 2 cases, a t(8;14)(q24;q32) and a t(9;14)(p13;q32) in 1 case 
      each. We also noted a t(14;17)(q32;q21) in 1 case. The use of FISH was a valuable 
      asset in determining the origin of the additional material on chromosome 14q32, 
      and helped resolve a group of B-cell NHLs with involvement of a duplicated 14q32 
      region.
FAU - Arcaroli, J J
AU  - Arcaroli JJ
AD  - Munroe Meyer Institute for Genetics and Rehabilitation, University of Nebraska 
      Medical Center, Omaha 68198-5440, USA.
FAU - Dave, B J
AU  - Dave BJ
FAU - Pickering, D L
AU  - Pickering DL
FAU - Hess, M M
AU  - Hess MM
FAU - Armitage, J O
AU  - Armitage JO
FAU - Weisenburger, D D
AU  - Weisenburger DD
FAU - Sanger, W G
AU  - Sanger WG
LA  - eng
GR  - CA36727/CA/NCI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Cancer Genet Cytogenet
JT  - Cancer genetics and cytogenetics
JID - 7909240
SB  - IM
MH  - *Chromosome Aberrations
MH  - *Chromosomes, Human, Pair 14
MH  - *Gene Duplication
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Lymphoma, B-Cell/*genetics
MH  - Retrospective Studies
EDAT- 1999/08/25 00:00
MHDA- 1999/08/25 00:01
CRDT- 1999/08/25 00:00
PHST- 1999/08/25 00:00 [pubmed]
PHST- 1999/08/25 00:01 [medline]
PHST- 1999/08/25 00:00 [entrez]
AID - S0165460898002866 [pii]
AID - 10.1016/s0165-4608(98)00286-6 [doi]
PST - ppublish
SO  - Cancer Genet Cytogenet. 1999 Aug;113(1):19-24. doi: 
      10.1016/s0165-4608(98)00286-6.