PMID- 10459343
OWN - NLM
STAT- MEDLINE
DCOM- 19990910
LR  - 20190816
IS  - 0165-4608 (Print)
IS  - 0165-4608 (Linking)
VI  - 113
IP  - 1
DP  - 1999 Aug
TI  - Detection of translocation 8;21 on interphase cells from acute myelocytic 
      leukemia by fluorescence in situ hybridization and its clinical application.
PG  - 29-35
AB  - To detect a translocation (8;21)(q22;q22) in interphase cells by fluorescence in 
      situ hybridization (FISH), we investigated three probe combinations: single-color 
      hybridization with two cosmid probes (cY8 and cY3), single-color hybridization 
      with four cosmid probes (cY8, cY3, cY107, and cYR4), and dual-color hybridization 
      with two cosmid probes (cY107 and cYR4) from the AML1 gene flanking or 
      overlapping the breakpoint region. Over 95% of nuclei gave sufficient signals in 
      all three probe combinations, and the detection rates were not statistically 
      different among them. Among 18 patients examined at diagnosis, 12 with t(8;21) 
      were also monitored for the number of residual leukemic cells after chemotherapy 
      or bone marrow transplantation (BMT). There were some discrepancies between 
      morphology and genetic (especially FISH) results at partial, or even complete 
      remission. As leukemic cells with t(8;21) can maturate, morphological assessment 
      alone is insufficient to evaluate the residual leukemic cells. Interphase FISH 
      provided more precise information about the clinical status of patients with an 
      8;21 translocation after treatment.
FAU - Tanaka, K
AU  - Tanaka K
AD  - Department of Cancer Cytogenetics, Hiroshima University, Japan.
FAU - Arif, M
AU  - Arif M
FAU - Asou, H
AU  - Asou H
FAU - Shimizu, K
AU  - Shimizu K
FAU - Ohki, M
AU  - Ohki M
FAU - Kyo, T
AU  - Kyo T
FAU - Dohy, H
AU  - Dohy H
FAU - Kamada, N
AU  - Kamada N
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Cancer Genet Cytogenet
JT  - Cancer genetics and cytogenetics
JID - 7909240
RN  - 0 (Core Binding Factor Alpha 2 Subunit)
RN  - 0 (DNA-Binding Proteins)
RN  - 0 (Proto-Oncogene Proteins)
RN  - 0 (RUNX1 protein, human)
RN  - 0 (Transcription Factors)
SB  - IM
MH  - Adult
MH  - *Chromosomes, Human, Pair 21
MH  - *Chromosomes, Human, Pair 8
MH  - Core Binding Factor Alpha 2 Subunit
MH  - *DNA-Binding Proteins
MH  - Humans
MH  - In Situ Hybridization, Fluorescence/*methods
MH  - Interphase
MH  - Leukemia, Myeloid, Acute/*genetics
MH  - Models, Genetic
MH  - *Proto-Oncogene Proteins
MH  - Time Factors
MH  - Transcription Factors/genetics
MH  - *Translocation, Genetic
EDAT- 1999/08/25 00:00
MHDA- 1999/08/25 00:01
CRDT- 1999/08/25 00:00
PHST- 1999/08/25 00:00 [pubmed]
PHST- 1999/08/25 00:01 [medline]
PHST- 1999/08/25 00:00 [entrez]
AID - S0165460899000114 [pii]
AID - 10.1016/s0165-4608(99)00011-4 [doi]
PST - ppublish
SO  - Cancer Genet Cytogenet. 1999 Aug;113(1):29-35. doi: 
      10.1016/s0165-4608(99)00011-4.