PMID- 10459857 OWN - NLM STAT- MEDLINE DCOM- 19991020 LR - 20190813 IS - 0303-7207 (Print) IS - 0303-7207 (Linking) VI - 153 IP - 1-2 DP - 1999 Jul 20 TI - The first exon of the human sc gene contains an androgen responsive unit and an interferon regulatory factor element. PG - 91-102 AB - Secretory component (SC) plays a key role in the transport of IgA and IgM to the lumina of many glands. The gene is constitutively expressed, but can be modulated by hormonal and immunological stimuli. Recently, the promoter and the first exon of the human sc gene have been cloned. The first exon contains a putative androgen/glucocorticoid response element (ARE/GRE) and an Interferon Regulatory Factor Element (IRF-E). Here we show that the ARE/GRE can bind the DNA-binding domain (DBD) of both the androgen (AR) and glucocorticoid receptor (GR) with a preference for the AR-DBD. In transient transfection experiments, this element confers higher responsiveness to androgens than to glucocorticoids. The IRF-E can function as an IRF-2, but surprisingly not as an IRF-I responsive element. We postulate that these two regulatory elements play a key role in the complex regulation of the sc gene in vivo. FAU - Haelens, A AU - Haelens A AD - Division of Biochemistry, Faculty of Medicine, University of Leuven, Belgium. FAU - Verrijdt, G AU - Verrijdt G FAU - Schoenmakers, E AU - Schoenmakers E FAU - Alen, P AU - Alen P FAU - Peeters, B AU - Peeters B FAU - Rombauts, W AU - Rombauts W FAU - Claessens, F AU - Claessens F LA - eng PT - Journal Article PT - Research Support, Non-U.S. Gov't PL - Ireland TA - Mol Cell Endocrinol JT - Molecular and cellular endocrinology JID - 7500844 RN - 0 (Androgens) RN - 0 (Glucocorticoids) RN - 0 (Receptors, Androgen) RN - 0 (Receptors, Glucocorticoid) RN - 0 (Secretory Component) RN - 9007-49-2 (DNA) RN - 9008-11-1 (Interferons) SB - IM MH - Androgens/*pharmacology MH - Animals MH - Base Sequence MH - Binding Sites MH - Breast Neoplasms MH - DNA/chemistry/metabolism MH - *Exons MH - Glucocorticoids/pharmacology MH - Humans MH - Interferons/*pharmacology MH - Molecular Sequence Data MH - Promoter Regions, Genetic MH - Rats MH - Receptors, Androgen/metabolism MH - Receptors, Glucocorticoid/metabolism MH - Regulatory Sequences, Nucleic Acid MH - *Response Elements MH - Secretory Component/*genetics MH - Transfection MH - Tumor Cells, Cultured EDAT- 1999/08/25 00:00 MHDA- 1999/08/25 00:01 CRDT- 1999/08/25 00:00 PHST- 1999/08/25 00:00 [pubmed] PHST- 1999/08/25 00:01 [medline] PHST- 1999/08/25 00:00 [entrez] AID - S0303-7207(99)00079-9 [pii] AID - 10.1016/s0303-7207(99)00079-9 [doi] PST - ppublish SO - Mol Cell Endocrinol. 1999 Jul 20;153(1-2):91-102. doi: 10.1016/s0303-7207(99)00079-9.