PMID- 10460352
OWN - NLM
STAT- MEDLINE
DCOM- 19991006
LR  - 20190921
IS  - 0939-5555 (Print)
IS  - 0939-5555 (Linking)
VI  - 78
IP  - 8
DP  - 1999 Aug
TI  - Hematopoietic donor chimerism and graft-versus-myeloma effect in relapse of 
      multiple myeloma after allogeneic bone marrow transplantation.
PG  - 376-9
AB  - A large group of patients relapsing after allogeneic bone marrow transplantation 
      (BMT) have obtained remission after infusion of leukocytes from their original 
      donor, suggesting a graft-versus-myeloma effect. However, side effects such as 
      graft-versus-host disease and myelosuppression are severe, and sometimes fatal, 
      complications of this therapeutic approach. Previously we demonstrated that 
      patients with leukemia who lack donor hematopoiesis in relapse after BMT 
      experience severe and lasting aplasia after infusion of donor leukocytes. In two 
      patients - one with extramedullary and one with marrow relapse after a 
      sex-mismatched transplantation - we analyzed hematopoietic chimerism by cell 
      sorting and bone marrow cultures. CD34-positive cells, CD4-CD8-positive cells, 
      committed progenitors, and LTC-IC were of donor origin, as demonstrated by 
      two-color fluorescence in situ hybridization (FISH). Additionally, in relapse 
      complete donor T-cell chimerism was seen. In contrast, plasma cells were of 
      recipient origin in the patient who had a relapse in the bone marrow. Both 
      patients were treated with infusions of donor leukocytes from their original 
      donor. Neither patient suffered myelosuppression, and one achieved a stable 
      complete remission.
FAU - Keil, F
AU  - Keil F
AD  - Department of Medicine 1, Bone Marrow Transplantation Unit, University of Vienna, 
      Austria. felix.keil@akh-wien.ac.at
FAU - Kalhs, P
AU  - Kalhs P
FAU - Chen, X
AU  - Chen X
FAU - Haas, O A
AU  - Haas OA
FAU - Fritsch, G
AU  - Fritsch G
FAU - Chott, A
AU  - Chott A
FAU - Lechner, K
AU  - Lechner K
FAU - Moser, K
AU  - Moser K
FAU - Ackermann, J
AU  - Ackermann J
FAU - Rabitsch, W
AU  - Rabitsch W
FAU - Worel, N
AU  - Worel N
FAU - Mannhalter, C
AU  - Mannhalter C
FAU - Greinix, H T
AU  - Greinix HT
LA  - eng
PT  - Case Reports
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Germany
TA  - Ann Hematol
JT  - Annals of hematology
JID - 9107334
RN  - 0 (Antigens, CD34)
SB  - IM
MH  - Adult
MH  - Antigens, CD34
MH  - Biopsy
MH  - Blood Donors
MH  - Bone Marrow/abnormalities/pathology
MH  - *Bone Marrow Transplantation/immunology
MH  - CD4-Positive T-Lymphocytes
MH  - CD8-Positive T-Lymphocytes/cytology
MH  - Female
MH  - Graft vs Host Disease/therapy
MH  - Graft vs Tumor Effect
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Leukapheresis
MH  - Male
MH  - Middle Aged
MH  - Multiple Myeloma/*therapy
MH  - Transplantation Chimera
MH  - Transplantation, Homologous
EDAT- 1999/08/25 00:00
MHDA- 1999/08/25 00:01
CRDT- 1999/08/25 00:00
PHST- 1999/08/25 00:00 [pubmed]
PHST- 1999/08/25 00:01 [medline]
PHST- 1999/08/25 00:00 [entrez]
AID - 90780376.277 [pii]
AID - 10.1007/s002770050532 [doi]
PST - ppublish
SO  - Ann Hematol. 1999 Aug;78(8):376-9. doi: 10.1007/s002770050532.