PMID- 10460495
OWN - NLM
STAT- MEDLINE
DCOM- 19991012
LR  - 20190720
IS  - 0302-766X (Print)
IS  - 0302-766X (Linking)
VI  - 297
IP  - 3
DP  - 1999 Sep
TI  - Localization and ultrastructure of pulmonary dendritic cells during delayed-type 
      hypersensitivity reactions in mice.
PG  - 485-92
AB  - Dendritic cells (DCs) are widely distributed in the airways and can serve as 
      potent antigen-presenting cells. To clarify their involvement in the 
      cell-mediated immune responses of the lung, we immunohistochemically investigated 
      their distribution and kinetics during pulmonary delayed-type hypersensitivity 
      (DTH) reactions induced in sensitized mice by intratracheal instillation of 
      hapten. Cellular infiltrate appeared around the bronchiole and its accompanying 
      blood vessel at 12 h after elicitation and progressively expanded by 48 h. As 
      quantitated by computer-assisted morphometry, I-A(+) DCs and CD4(+) Th cells 
      significantly increased in number around the bronchiole to a maximum at 24 h, 
      whereas F4/80(+) macrophages were predominantly accumulated around the 
      accompanying vessel with a peak at 48 h. Serial-section analysis revealed that 
      DCs were colocalized with Th cells in the inflamed peribronchiolar tissue. 
      Immunoelectron microscopy demonstrated that DCs found inside and around the 
      capillaries and venules of peribronchiolar interstitium displayed round forms, 
      indicating their emigration from here, while those situated far from the 
      microvessels were elongated, often in close apposition to the lymphocytes. 
      Mitosis of DCs was rarely seen. The present results suggest that peribronchiolar 
      accumulation of DCs resulting from accelerated influx of blood-borne immature DCs 
      and the interaction with T cells at the application site may play inducing roles 
      in the development of pulmonary DTH reactions by enhancing the recruitment of 
      macrophages.
FAU - Nishida, T
AU  - Nishida T
AD  - Department of Anatomy, Osaka City University Medical School, 1-4-3 Asahimachi, 
      Abeno-ku, Osaka 545-8585, Japan. nishidat@msic.med.osaka-cu.ac.jp
FAU - Inoue, K
AU  - Inoue K
FAU - Kato, T
AU  - Kato T
FAU - Iwata, T
AU  - Iwata T
FAU - Kinoshita, H
AU  - Kinoshita H
FAU - Kaneda, K
AU  - Kaneda K
LA  - eng
PT  - Journal Article
PL  - Germany
TA  - Cell Tissue Res
JT  - Cell and tissue research
JID - 0417625
SB  - IM
MH  - Animals
MH  - Dendritic Cells/immunology/pathology/*ultrastructure
MH  - Female
MH  - Hypersensitivity, Delayed/immunology/*pathology
MH  - Image Cytometry
MH  - Lung/immunology/*pathology/ultrastructure
MH  - Mice
MH  - Mice, Inbred BALB C
MH  - Microscopy, Electron
EDAT- 1999/08/25 00:00
MHDA- 1999/08/25 00:01
CRDT- 1999/08/25 00:00
PHST- 1999/08/25 00:00 [pubmed]
PHST- 1999/08/25 00:01 [medline]
PHST- 1999/08/25 00:00 [entrez]
AID - 92970485.441 [pii]
AID - 10.1007/s004410051375 [doi]
PST - ppublish
SO  - Cell Tissue Res. 1999 Sep;297(3):485-92. doi: 10.1007/s004410051375.