PMID- 10460754
OWN - NLM
STAT- MEDLINE
DCOM- 19991019
LR  - 20220224
IS  - 1044-1549 (Print)
IS  - 1044-1549 (Linking)
VI  - 21
IP  - 3
DP  - 1999 Sep
TI  - Antigen trafficking and accessory cell function in respiratory epithelial cells.
PG  - 365-79
AB  - We investigated accessory cell function, antigen (Ag) trafficking, and uptake of 
      immune complexes in isolated nasal epithelial cells (NEC) and airway epithelial 
      cells (AEC), as well as in the two respiratory epithelial cell lines A549 and 
      BEAS-2B. The NEC and AEC were capable of supporting Ag-specific as well as 
      phytohemagglutinin-induced and anti-CD3 antibody-induced T-cell proliferation. We 
      colocalized fluorescein isothiocyanate (FITC)-labeled Ags with human leukocyte 
      antigen (HLA)-DR in A549 and BEAS-2B, utilizing laser confocal microscopy. 
      Respiratory epithelial cells stimulated and unstimulated with interferon 
      (IFN)-gamma were pulsed with FITC-labeled Ags for varying periods and evaluated 
      for their ability to internalize Ag. In the unstimulated cells, intracellular 
      punctate staining was evident at 60 min and persisted up to 120 min. In the 
      IFN-gamma-stimulated cells (100 U/ml for 48 h), uptake occurred at 30 min, was 
      maximal at 60 min, and diminished at 120 min. We conducted kinetic studies in the 
      A549 and BEAS-2B cells, utilizing electron microscopy with colloidal 
      gold-conjugated Ags (Au-OVA). At 15 min, Au-OVA was evident in the early 
      compartments resembling the compartment of uncoupling of receptor and ligand. At 
      30 min, multivesicular bodies were labeled with Au-OVA, and by 60 min Au-OVA was 
      present in the primary and secondary lysosomes. The FITC-labeled Ags colocalized 
      with an early endosomal marker (anti-cathepsin D), a late endosomal marker 
      (M6PR), a lysosomal marker (CD63), and with 3-(2, 
      4-dinitroanilino)-3'-aminomethyldipropylamine, a marker of acidic vesicles. The 
      BEAS-2B and A549 cells, and NEC and AEC, expressed surface Fcgamma receptor and 
      internalized IgG immune complexes. The NEC and AEC also expressed the 
      costimulatory molecules CD80 and CD86 as determined with flow cytometry, the 
      reverse transcription-polymerase chain reaction for RNA, and 
      immunohistochemistry, and T-cell proliferation could be blocked by treating NEC 
      and AEC with anti-CD80 and anti-CD86 antibodies. Our findings suggest that 
      respiratory epithelial cells may have a role in local Ag presentation.
FAU - Salik, E
AU  - Salik E
AD  - Divisions of Clinical Immunology and Pulmonary and Critical Care Medicine, Mount 
      Sinai Medical Center, New York City, New York, USA.
FAU - Tyorkin, M
AU  - Tyorkin M
FAU - Mohan, S
AU  - Mohan S
FAU - George, I
AU  - George I
FAU - Becker, K
AU  - Becker K
FAU - Oei, E
AU  - Oei E
FAU - Kalb, T
AU  - Kalb T
FAU - Sperber, K
AU  - Sperber K
LA  - eng
GR  - 1S1ORR09145-01/OR/ORS NIH HHS/United States
GR  - R29CA66522/CA/NCI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, Non-P.H.S.
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Am J Respir Cell Mol Biol
JT  - American journal of respiratory cell and molecular biology
JID - 8917225
RN  - 0 (Antigen-Antibody Complex)
RN  - 0 (Antigens)
RN  - 0 (Antigens, CD)
RN  - 0 (B7-1 Antigen)
RN  - 0 (B7-2 Antigen)
RN  - 0 (CD3 Complex)
RN  - 0 (CD86 protein, human)
RN  - 0 (HLA-DR Antigens)
RN  - 0 (Membrane Glycoproteins)
RN  - 0 (Receptors, IgG)
SB  - IM
MH  - Antigen-Antibody Complex/immunology
MH  - Antigen-Presenting Cells/immunology
MH  - Antigens/*immunology
MH  - Antigens, CD/immunology
MH  - B7-1 Antigen/immunology
MH  - B7-2 Antigen
MH  - Bronchi/*immunology/*physiology
MH  - CD3 Complex/immunology
MH  - Cell Line
MH  - Cells, Cultured
MH  - Endocytosis
MH  - Epithelial Cells/immunology
MH  - HLA-DR Antigens/metabolism
MH  - Humans
MH  - Kinetics
MH  - Lymphocyte Activation/drug effects
MH  - Membrane Glycoproteins/immunology
MH  - Microscopy, Confocal
MH  - Microscopy, Electron
MH  - Nasal Mucosa/*immunology/*physiology
MH  - Receptors, IgG/metabolism
MH  - Reverse Transcriptase Polymerase Chain Reaction
MH  - Time Factors
EDAT- 1999/08/26 00:00
MHDA- 1999/08/26 00:01
CRDT- 1999/08/26 00:00
PHST- 1999/08/26 00:00 [pubmed]
PHST- 1999/08/26 00:01 [medline]
PHST- 1999/08/26 00:00 [entrez]
AID - 10.1165/ajrcmb.21.3.3529 [doi]
PST - ppublish
SO  - Am J Respir Cell Mol Biol. 1999 Sep;21(3):365-79. doi: 10.1165/ajrcmb.21.3.3529.