PMID- 10461681
OWN - NLM
STAT- MEDLINE
DCOM- 19990930
LR  - 20031114
IS  - 1078-0297 (Print)
IS  - 1078-0297 (Linking)
VI  - 103
IP  - 2
DP  - 1999 Feb
TI  - Neutrophil elastase enhances macrophage production of chemokines in 
      receptor-mediated reaction.
PG  - 139-47
AB  - Proteases influence various leukocyte effector functions. We investigated the 
      specific binding activity of neutrophil elastase (NE) on rat peritoneal 
      macrophages to stimulate chemokine production in vitro. NE enhanced macrophage 
      production of monocyte chemo-attractant protein-1 (MCP-1) (CC-chemokine) and 
      cytokine-induced neutrophil chemo-attractant (CINC) (CXC-chemokine). However, the 
      serine protease inhibitor, phenylmethylsulfonyl fluoride (PMSF), significantly 
      reduced these chemokine productions by macrophages stimulated with NE. 125I-NE 
      was significantly bound to macrophages, but 125I-NE binding was inhibited by 
      addition of unlabeled NE. In addition, NE significantly stimulated [3H]thymidine 
      incorporation into DNA on 3T3 fibroblasts in a dose-dependent manner. These 
      results suggest that NE stimulates chemokine production by macrophages. This may 
      be a receptor-mediated reaction. Thus, NE not only degrades extra-cellular matrix 
      but also stimulates chemokine production by macrophages.
FAU - Ishihara, K
AU  - Ishihara K
AD  - Department of Surgery II, Kumamoto University Medical School, Japan.
FAU - Yamaguchi, Y
AU  - Yamaguchi Y
FAU - Okabe, K
AU  - Okabe K
FAU - Ogawa, M
AU  - Ogawa M
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Res Commun Mol Pathol Pharmacol
JT  - Research communications in molecular pathology and pharmacology
JID - 9437512
RN  - 0 (Chemokines)
RN  - 0 (Receptors, Cell Surface)
RN  - EC 3.4.21.37 (Leukocyte Elastase)
SB  - IM
MH  - 3T3 Cells
MH  - Animals
MH  - Chemokines/*biosynthesis
MH  - Leukocyte Elastase/*metabolism
MH  - Macrophages, Peritoneal/*metabolism
MH  - Male
MH  - Mice
MH  - Rats
MH  - Rats, Wistar
MH  - Receptors, Cell Surface/*metabolism
EDAT- 1999/08/26 00:00
MHDA- 1999/08/26 00:01
CRDT- 1999/08/26 00:00
PHST- 1999/08/26 00:00 [pubmed]
PHST- 1999/08/26 00:01 [medline]
PHST- 1999/08/26 00:00 [entrez]
PST - ppublish
SO  - Res Commun Mol Pathol Pharmacol. 1999 Feb;103(2):139-47.