PMID- 10461923
OWN - NLM
STAT- MEDLINE
DCOM- 19990914
LR  - 20190630
IS  - 0022-3042 (Print)
IS  - 0022-3042 (Linking)
VI  - 73
IP  - 3
DP  - 1999 Sep
TI  - Cysteine 144 is a key residue in the copper reduction by the beta-amyloid 
      precursor protein.
PG  - 1288-92
AB  - The beta-amyloid precursor protein (beta-APP) contains a copper-binding site 
      localized between amino acids 135 and 156 (beta-APP(135-156)). We have employed 
      synthetic beta-APP peptides to characterize their capacities to reduce Cu(II) to 
      Cu(I). Analogues of the wild-type beta-APP(135-156) peptide, containing specific 
      amino acid substitutions, were used to establish which residues are specifically 
      involved in the reduction of copper by beta-APP(135-156). We report here that 
      beta-APP's copper-binding domain reduced Cu(II) to Cu(I). The single-mutant 
      beta-APP(His147-->Ala) and the double-mutant beta-APP(His147-->Ala/His149-->Ala) 
      showed a small decrease in copper reduction in relation to the wild-type peptide 
      and the beta-APP(Cys144-->Ser) mutation abolished it, suggesting that Cys144 is 
      the key amino acid in the oxidoreduction reaction. Our results confirm that 
      soluble beta-APP is involved in the reduction of Cu(II) to Cu(I).
FAU - Ruiz, F H
AU  - Ruiz FH
AD  - Departamento de Biologia Celular y Molecular, Facultad de Ciencias Biologicas, 
      Santiago, Chile.
FAU - Gonzalez, M
AU  - Gonzalez M
FAU - Bodini, M
AU  - Bodini M
FAU - Opazo, C
AU  - Opazo C
FAU - Inestrosa, N C
AU  - Inestrosa NC
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - J Neurochem
JT  - Journal of neurochemistry
JID - 2985190R
RN  - 0 (Amyloid beta-Protein Precursor)
RN  - 0 (Peptide Fragments)
RN  - 789U1901C5 (Copper)
RN  - K848JZ4886 (Cysteine)
SB  - IM
MH  - Amino Acid Substitution
MH  - Amyloid beta-Protein Precursor/*chemistry
MH  - Copper/*chemistry
MH  - Cysteine/*chemistry
MH  - Humans
MH  - Mutation
MH  - Oxidation-Reduction
MH  - Peptide Fragments/chemistry
MH  - Potentiometry
EDAT- 1999/08/26 00:00
MHDA- 1999/08/26 00:01
CRDT- 1999/08/26 00:00
PHST- 1999/08/26 00:00 [pubmed]
PHST- 1999/08/26 00:01 [medline]
PHST- 1999/08/26 00:00 [entrez]
AID - 10.1046/j.1471-4159.1999.0731288.x [doi]
PST - ppublish
SO  - J Neurochem. 1999 Sep;73(3):1288-92. doi: 10.1046/j.1471-4159.1999.0731288.x.