PMID- 10462494
OWN - NLM
STAT- MEDLINE
DCOM- 19991001
LR  - 20161019
IS  - 0006-291X (Print)
IS  - 0006-291X (Linking)
VI  - 262
IP  - 2
DP  - 1999 Aug 27
TI  - Mouse phosphoinositide 3-kinase p110alpha gene: cloning, structural organization, 
      and localization to chromosome 3 band B.
PG  - 438-42
AB  - Phosphoinositide 3-Kinases (PI3-Kinases) are a family of dual specificity enzymes 
      with a unique lipid kinase activity toward the D-3 position of the inositol ring 
      of phosphoinositides and a less well characterized serine/threonine protein 
      kinase activity. Class IA PI3-Kinases comprise a 110-120 kDa catalytic subunit 
      (usually termed p110) and an 85 kDa or 50 to 55 kDa regulatory subunit (often 
      called p85). cDNAs for three mammalian Class IA PI3-Kinase catalytic subunits 
      designated p110alpha, p110beta, and p110delta have been cloned from several 
      species. A YAC clone for the human p110alpha gene has also been cloned and mapped 
      to chromosome 3q26.3. However, structural organization for any of the PI3-Kinase 
      p110alpha genes has not been reported. Here, we report the cloning, structural 
      organization, and chromosomal localization of the mouse PI3-Kinase p110alpha 
      gene. The translated portion of the mouse p110alpha gene is encoded by 19 exons 
      that span at least 24 kb. Dual color fluorescence in situ hybridization (FISH) 
      was performed to determine the chromosomal localization of the mouse PI3-Kinase 
      p110alpha gene. FISH results and DAPI banding demonstrated localization of the 
      p110alpha gene to band B on mouse chromosome 3, a region syntenic with human 
      chromosome 3q26.3.
CI  - Copyright 1999 Academic Press.
FAU - Aksoy, I A
AU  - Aksoy IA
AD  - Dana Farber Cancer Institute, Harvard Medical School, Boston, Massachusetts, 
      02115, USA.
FAU - Ramsey, M J
AU  - Ramsey MJ
FAU - Fruman, D A
AU  - Fruman DA
FAU - Aksoy, S
AU  - Aksoy S
FAU - Cantley, L C
AU  - Cantley LC
FAU - Tucker, J D
AU  - Tucker JD
FAU - Roberts, T M
AU  - Roberts TM
LA  - eng
GR  - R01 GM041890/GM/NIGMS NIH HHS/United States
GR  - CA 73155/CA/NCI NIH HHS/United States
GR  - CA30002/CA/NCI NIH HHS/United States
GR  - GM41890/GM/NIGMS NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, Non-P.H.S.
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Biochem Biophys Res Commun
JT  - Biochemical and biophysical research communications
JID - 0372516
RN  - EC 2.7.1.- (Phosphatidylinositol 3-Kinases)
SB  - IM
MH  - Animals
MH  - Base Sequence
MH  - Chromosome Mapping
MH  - Exons
MH  - Genes
MH  - Genomic Library
MH  - In Situ Hybridization, Fluorescence
MH  - Introns
MH  - Mice/*genetics
MH  - Molecular Sequence Data
MH  - Phosphatidylinositol 3-Kinases/*genetics
MH  - Polymerase Chain Reaction
MH  - RNA Splicing
MH  - Sequence Analysis, DNA
EDAT- 1999/08/27 00:00
MHDA- 1999/08/27 00:01
CRDT- 1999/08/27 00:00
PHST- 1999/08/27 00:00 [pubmed]
PHST- 1999/08/27 00:01 [medline]
PHST- 1999/08/27 00:00 [entrez]
AID - S0006-291X(99)91150-6 [pii]
AID - 10.1006/bbrc.1999.1150 [doi]
PST - ppublish
SO  - Biochem Biophys Res Commun. 1999 Aug 27;262(2):438-42. doi: 
      10.1006/bbrc.1999.1150.