PMID- 10462574
OWN - NLM
STAT- Publisher
LR  - 20191120
IS  - 1084-8592 (Print)
IS  - 1084-8592 (Linking)
VI  - 1
IP  - 4
DP  - 1996 Dec
TI  - Identification of MYCN Copy Number Heterogeneity by Direct FISH Analysis of 
      Neuroblastoma Preparations.
PG  - 281-289
AB  - Background: Amplification of MYCN and deletion of 1p in neuroblastoma are 
      associated wtih a poor prognosis. MYCN copy numbers of 10 or greater are 
      considered to be indicative of poor outcome. However, most molecular genetic 
      methods for estimating the number of MYCN genes do not directly address copy 
      number heterogeneity at the cellular level. Methods and Results: MYCN copy number 
      was assessed by fluorescence in situ hybridization (FISH), differential 
      polymerase chain reaction (PCR), and Southern blot analysis, using 29 patient 
      tumors. Copy number estimates by PCR or Southern blot analyses identified MYCN 
      amplification in 11 tumors. There was complete concordance between FISH MYCN 
      results in all 11 tumors with amplification. FISH identified 5 tumors with marked 
      heterogeneity for MYCN copy number. Two tumors in which a small percentage of 
      cells within the specimen were amplified would have gone undetected by molecular 
      genetic methods alone. Conclusions: FISH offers the advantage over the other 
      methods of detecting heterogeneity, thereby revealing tumors with small numbers 
      of amplified cells that would otherwise be missed and, in cases of low (3-10) 
      copies of MYCN, distinguishing small numbers of amplified cells (poor prognosis) 
      from triploid tumors (good prognosis). FISH also allows detection of 1p deletion 
      using the same preparations, which represents another advantage. A combination of 
      FISH and conventional molecular methods provides an accurate definition of sample 
      heterogeneity, and should be routinely applied to all neuroblastomas with low 
      levels of MYCN copy number.
FAU - Squire, JA
AU  - Squire JA
AD  - Department of Pediatric Laboratory Medicine (Division of Pathology), The Hospital 
      for Sick Children, Toronto, Canada
FAU - Thorner, P
AU  - Thorner P
FAU - Marrano, P
AU  - Marrano P
FAU - Parkinson, D
AU  - Parkinson D
FAU - Ng, YK
AU  - Ng YK
FAU - Gerrie, B
AU  - Gerrie B
FAU - Chilton-Macneill, S
AU  - Chilton-Macneill S
FAU - Zielenska, M
AU  - Zielenska M
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Mol Diagn
JT  - Molecular diagnosis : a journal devoted to the understanding of human disease 
      through the clinical application of molecular biology
JID - 9614965
EDAT- 1996/12/01 00:00
MHDA- 1999/08/27 00:00
CRDT- 1996/12/01 00:00
PHST- 1996/12/01 00:00 [pubmed]
PHST- 1999/08/27 00:00 [medline]
PHST- 1996/12/01 00:00 [entrez]
AID - S1084859296000215 [pii]
AID - 10.1054/MODI00100281 [doi]
PST - ppublish
SO  - Mol Diagn. 1996 Dec;1(4):281-289. doi: 10.1054/MODI00100281.