PMID- 10462722
OWN - NLM
STAT- MEDLINE
DCOM- 19990921
LR  - 20191024
IS  - 0893-6692 (Print)
IS  - 0893-6692 (Linking)
VI  - 34
IP  - 1
DP  - 1999
TI  - Aneuploidy in sperm and exposure to fungicides and lifestyle factors. ASCLEPIOS. 
      A European Concerted Action on Occupational Hazards to Male Reproductive 
      Capability.
PG  - 39-46
AB  - Fungicides include chemicals that are known aneugens. The purpose of the present 
      study was to investigate whether occupational exposure to these and other 
      agricultural pesticides induces aneuploidy in human sperm. The contribution of 
      lifestyle factors (smoking and alcohol consumption) to the frequency of aneuploid 
      sperm was evaluated as well. The effects of age and sperm concentration were 
      analyzed as confounders. Spermatozoa from 30 healthy farmers were studied before 
      and after exposure to fungicides, using fluorescence in situ hybridization 
      (FISH). Ten thousand spermatozoa were scored per semen sample to determine the 
      disomy and diploidy frequencies for chromosomes 1 and 7. Exposure to fungicides 
      was not associated with sperm aneuploidy. Smoking was significantly associated 
      with sperm carrying an extra chromosome 1 and with diploid sperm as well as with 
      the aggregate frequency of aneuploid sperm. Alcohol consumption, sperm 
      concentration, and age showed inconsistent results before and after the season of 
      exposure to fungicides. For low-level exposures, such as occupational exposures, 
      the sensitivity of the sperm-FISH method may not be sufficient. The present study 
      supports earlier ones showing that smoking can increase aneuploidy in human 
      sperm.
CI  - Copyright 1999 Wiley-Liss, Inc.
FAU - Harkonen, K
AU  - Harkonen K
AD  - Department of Medical Genetics, University of Turku, Turku, Finland. 
      kajohar@utu.fi
FAU - Viitanen, T
AU  - Viitanen T
FAU - Larsen, S B
AU  - Larsen SB
FAU - Bonde, J P
AU  - Bonde JP
FAU - Lahdetie, J
AU  - Lahdetie J
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Environ Mol Mutagen
JT  - Environmental and molecular mutagenesis
JID - 8800109
RN  - 0 (Fungicides, Industrial)
SB  - IM
MH  - Adult
MH  - Age Factors
MH  - Agricultural Workers' Diseases/etiology/genetics/prevention & control
MH  - Alcohol Drinking
MH  - *Aneuploidy
MH  - Chromosomes, Human, Pair 1/genetics
MH  - Chromosomes, Human, Pair 7/genetics
MH  - Data Interpretation, Statistical
MH  - Europe
MH  - Fungicides, Industrial/*adverse effects
MH  - Genital Diseases, Male/etiology/genetics/prevention & control
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - *Life Style
MH  - Longitudinal Studies
MH  - Male
MH  - Middle Aged
MH  - Occupational Exposure/*adverse effects
MH  - Semen/drug effects/metabolism
MH  - Smoking
MH  - Sperm Count
MH  - Spermatozoa/*drug effects/metabolism
EDAT- 1999/08/27 00:00
MHDA- 1999/08/27 00:01
CRDT- 1999/08/27 00:00
PHST- 1999/08/27 00:00 [pubmed]
PHST- 1999/08/27 00:01 [medline]
PHST- 1999/08/27 00:00 [entrez]
AID - 10.1002/(SICI)1098-2280(1999)34:1<39::AID-EM6>3.0.CO;2-F [pii]
AID - 10.1002/(sici)1098-2280(1999)34:1<39::aid-em6>3.0.co;2-f [doi]
PST - ppublish
SO  - Environ Mol Mutagen. 1999;34(1):39-46. doi: 
      10.1002/(sici)1098-2280(1999)34:1<39::aid-em6>3.0.co;2-f.