PMID- 10462729
OWN - NLM
STAT- MEDLINE
DCOM- 19991109
LR  - 20061115
IS  - 0897-3806 (Print)
IS  - 0897-3806 (Linking)
VI  - 12
IP  - 5
DP  - 1999
TI  - Spatial and temporal distribution of cellular proliferation in the cranial base 
      of normal and midfacially retrusive mice.
PG  - 315-25
AB  - The craniofacial region of the Brachyrrhine (Br) mouse is characterized by a 
      retruded midface. The cellular mechanism causing this growth deficiency is 
      unknown. However, the cranial base is foreshortened in adult Br mice. The purpose 
      of this study was to determine whether the spatial and temporal patterns of 
      cellular proliferation in the cranial base (CB) differ between normal (C3H/HeJ) 
      and Br mutant (3H1 Br/+) embryonic mice. Twenty-four dams were injected (3)H 
      thymidine (5 microCi/gram body weight) and 15 embryos from each group were 
      collected at Theiler stages 23, 25, and 27 (15, 17, and 19 days of gestation). 
      Serial sections from each head were processed with routine autoradiography. 
      Labelling indices (LI) were determined for each specimen and cellular 
      proliferation maps were generated for each age group. LI patterns within and 
      between groups were compared statistically. Results showed that cellular 
      proliferation in the CB of normal embryos displayed a time- and 
      position-dependent pattern, characteristic of transient growth sites (TGS). 
      Generally, as age increases, cellular proliferative activities decrease gradually 
      (from an average LI of 11.4 +/- 5.7% at stage 23 to 4.4 +/- 2.2% at stage 27), 
      and the number of the TGS decreases in the presumptive nasal septal region and 
      increases in presumptive sphenoethmoidal area with age, indicating the existence 
      of cellular subpopulations in the CB. Cellular proliferation in the CB of the Br 
      mutant displays a different growth pattern compared to the normal condition. 
      Deficiencies in cellular proliferation exist mainly in the presumptive 
      sphenoethmoidal area of the CB. The results indicate that the TGS play an 
      important role in the normal morphogenesis of the CB, and abnormalities in their 
      timing and/or position may be responsible for the dysmorphology of the midface in 
      the Br mutant.
CI  - Copyright 1999 Wiley-Liss, Inc.
FAU - Ma, W
AU  - Ma W
AD  - Department of Anatomy and Reproductive Biology, John A. Burns School of Medicine, 
      University of Hawaii, Honolulu, Hawaii 96822, USA.
FAU - Lozanoff, S
AU  - Lozanoff S
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Clin Anat
JT  - Clinical anatomy (New York, N.Y.)
JID - 8809128
SB  - IM
MH  - Animals
MH  - Apoptosis
MH  - Autoradiography
MH  - Cartilage/embryology/pathology
MH  - Cell Division
MH  - Craniofacial Abnormalities/*embryology/genetics/pathology
MH  - Ethmoid Bone/embryology/pathology
MH  - Female
MH  - Mice
MH  - Mice, Inbred C3H/embryology
MH  - Mice, Mutant Strains/embryology
MH  - Pregnancy
MH  - Skull Base/*embryology/pathology
MH  - Sphenoid Bone/embryology/pathology
EDAT- 1999/08/27 00:00
MHDA- 1999/08/27 00:01
CRDT- 1999/08/27 00:00
PHST- 1999/08/27 00:00 [pubmed]
PHST- 1999/08/27 00:01 [medline]
PHST- 1999/08/27 00:00 [entrez]
AID - 10.1002/(SICI)1098-2353(1999)12:5<315::AID-CA2>3.0.CO;2-2 [pii]
AID - 10.1002/(SICI)1098-2353(1999)12:5<315::AID-CA2>3.0.CO;2-2 [doi]
PST - ppublish
SO  - Clin Anat. 1999;12(5):315-25. doi: 
      10.1002/(SICI)1098-2353(1999)12:5<315::AID-CA2>3.0.CO;2-2.