PMID- 10464651
OWN - NLM
STAT- MEDLINE
DCOM- 19991012
LR  - 20091119
IS  - 1090-6576 (Print)
IS  - 1090-6576 (Linking)
VI  - 1
IP  - 4
DP  - 1997-1998
TI  - Prepregnancy genetic testing for age-related aneuploidies by polar body analysis.
PG  - 231-5
AB  - Current practice for prevention of chromosomal aneuploidies involves prenatal 
      screening and termination of pregnancy, a procedure that is not universally 
      acceptable. We introduced prepregnancy genetic testing by sampling and 
      fluorescence in situ hybridization (FISH) analysis of the first and second polar 
      body (PB), to avoid fertilization and transfer of embryos resulting from 
      aneuploid oocytes. In 395 in vitro fertilization (IVF) patients of advanced 
      maternal age, the first and second PBs were removed following their extrusion 
      from oocytes and studied by FISH, using probes specific for chromosomes 13, 18, 
      and 21, to detect and avoid the transfer of oocytes with common aneuploidies. 
      Overall, 3,651 oocytes obtained from 598 IVF cycles were available for FISH 
      analysis, with 2,952 showing interpretable FISH results (80.9%). The analysis 
      revealed 1,271 (43.1%) oocytes with aneuploidy, which were excluded from transfer 
      and subjected to follow-up FISH analysis to confirm PB diagnosis in the cleavage 
      or blastocyst stage embryos. Only embryos originating from 1,681 aneuploidy-free 
      oocytes were transferred back to patients, resulting in 119 pregnancies overall, 
      from which 78 healthy children have already been born, 35 were spontaneously 
      aborted, and 16 are ongoing, after confirming PB diagnosis by prenatal diagnosis. 
      The results demonstrate that PB-based preimplantation diagnosis may be used for 
      prepregnancy screening in women with age-related risk for common aneuploidies.
FAU - Verlinsky, Y
AU  - Verlinsky Y
AD  - Reproductive Genetics Institute, Chicago, IL 60657, USA.
FAU - Cieslak, J
AU  - Cieslak J
FAU - Ivakhnenko, V
AU  - Ivakhnenko V
FAU - Evsikov, S
AU  - Evsikov S
FAU - Wolf, G
AU  - Wolf G
FAU - White, M
AU  - White M
FAU - Lifchez, A
AU  - Lifchez A
FAU - Kaplan, B
AU  - Kaplan B
FAU - Moise, J
AU  - Moise J
FAU - Valle, J
AU  - Valle J
FAU - Ginsberg, N
AU  - Ginsberg N
FAU - Strom, C
AU  - Strom C
FAU - Kuliev, A
AU  - Kuliev A
LA  - eng
PT  - Journal Article
PT  - Review
PL  - United States
TA  - Genet Test
JT  - Genetic testing
JID - 9802546
SB  - IM
MH  - *Aneuploidy
MH  - Blastocyst
MH  - Embryo Transfer
MH  - Female
MH  - Fertilization in Vitro
MH  - *Genetic Testing
MH  - Humans
MH  - *In Situ Hybridization, Fluorescence
MH  - Infant, Newborn
MH  - *Maternal Age
MH  - *Meiosis
MH  - Oocytes/cytology
MH  - *Preconception Care
MH  - Pregnancy
MH  - Pregnancy Outcome
RF  - 13
EDAT- 1997/01/01 00:00
MHDA- 1999/08/28 00:01
CRDT- 1997/01/01 00:00
PHST- 1997/01/01 00:00 [pubmed]
PHST- 1999/08/28 00:01 [medline]
PHST- 1997/01/01 00:00 [entrez]
AID - 10.1089/gte.1997.1.231 [doi]
PST - ppublish
SO  - Genet Test. 1997-1998;1(4):231-5. doi: 10.1089/gte.1997.1.231.