PMID- 10465360
OWN - NLM
STAT- MEDLINE
DCOM- 19990916
LR  - 20190910
IS  - 0955-3002 (Print)
IS  - 0955-3002 (Linking)
VI  - 75
IP  - 8
DP  - 1999 Aug
TI  - Non-proportional involvement of Chinese hamster chromosomes 3, 4, 8 and 9 in 
      X-ray-induced chromosomal aberrations.
PG  - 943-51
AB  - PURPOSE: To study by fluorescence in situ hybridization (FISH) the involvement of 
      Chinese hamster chromosomes 3, 4, 8, and 9, and their separate chromosome arms in 
      X-ray-induced aberrations. MATERIALS AND METHODS: Male embryonic primary cells of 
      Chinese hamster were used and metaphases were collected and scored at 20 h after 
      confluent cells were exposed to 1 and 4 Gy X-rays. The frequencies and types of 
      chromosomal aberrations involving chromosomes 3, 4, 8, and 9 were studied by FISH 
      using arm-specific painting probes. Proportional distribution of the induced 
      aberrations was tested on the basis of the relative lengths of chromosomes or 
      chromosome arms studied. RESULTS: A non-proportional distribution of breaks, 
      colour junctions as well as apparently simple dicentrics and translocations among 
      the chromosomes studied was observed in the 4 Gy group. In general, chromosome 3 
      was less involved than expected and chromosome 8 was more involved than expected, 
      and chromosomes 4 and 9 were involved as expected. A non-proportional involvement 
      of arms in breaks, colour junctions and apparently simple dicentrics was also 
      observed. The short arm of chromosome 3 was more involved in breaks and colour 
      junctions than expected. The long arm of chromosome 4 was more involved in 
      dicentrics than expected. CONCLUSIONS: Results of this study indicate a 
      non-proportional involvement of Chinese hamster chromosomes 3, 4, 8 and 9 as well 
      as their arms in different types of aberrations following irradiation.
FAU - Xiao, Y
AU  - Xiao Y
AD  - Department of Radiation Genetics and Chemical Mutagenesis, Leiden University 
      Medical Centre, The Netherlands.
FAU - Natarajan, A T
AU  - Natarajan AT
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Int J Radiat Biol
JT  - International journal of radiation biology
JID - 8809243
RN  - 0 (DNA Probes)
SB  - IM
MH  - Animals
MH  - Cells, Cultured
MH  - *Chromosome Aberrations
MH  - Chromosome Fragility
MH  - Chromosomes/*radiation effects
MH  - Cricetinae
MH  - Cricetulus
MH  - DNA Damage
MH  - DNA Probes
MH  - Embryo, Mammalian
MH  - In Situ Hybridization, Fluorescence
MH  - Male
MH  - Radiation Dosage
EDAT- 2001/02/07 11:00
MHDA- 2001/02/07 11:01
CRDT- 2001/02/07 11:00
PHST- 2001/02/07 11:00 [pubmed]
PHST- 2001/02/07 11:01 [medline]
PHST- 2001/02/07 11:00 [entrez]
AID - 10.1080/095530099139700 [doi]
PST - ppublish
SO  - Int J Radiat Biol. 1999 Aug;75(8):943-51. doi: 10.1080/095530099139700.