PMID- 10467401
OWN - NLM
STAT- MEDLINE
DCOM- 19991004
LR  - 20161124
IS  - 0950-9232 (Print)
IS  - 0950-9232 (Linking)
VI  - 18
IP  - 32
DP  - 1999 Aug 12
TI  - Induction of mammary gland hyperplasia in transgenic mice over-expressing human 
      Cdc25B.
PG  - 4564-76
AB  - Cdc25 A and B are dual-specificity phosphatases which have been implicated in 
      neoplastic transformation. Although Cdc25A and Cdc25B have been found to be 
      over-expressed in many cancer cell lines and primary tumors, the physiological 
      roles of Cdc25A and B in vivo are largely undefined. To investigate the roles of 
      these proteins in the oncogenic transformation of the mammary gland we used the 
      mouse mammary tumor virus (MMTV) promoter to target over-expression of the Cdc25B 
      transgene in the mammary glands of transgenic mouse lines. Here we report that 
      the over-expression of Cdc25B enhances the proliferation of mammary epithelial 
      cells resulting in the formation of precocious alveolar hyperplasia. At the 
      molecular level, marked increases in cyclin D1 protein have been found in 
      transgenic mammary epithelial cells. The accelerated growth rate of the mammary 
      epithelial cells could also be attributed to the increased levels of cyclin 
      E/cdk2 activity. In addition, a pronounced decrease in apoptosis was also 
      observed during the involution of mammary gland. The reduction of apoptosis 
      during involution correlated well with the reduced expression of c-myc and p53, 
      both of which have been implicated in apoptosis. Taken together, our results 
      clearly indicate that the deregulated expression of Cdc25B generates mammary 
      gland hyperplasia.
FAU - Ma, Z Q
AU  - Ma ZQ
AD  - Department of Cell Biology, Baylor College of Medicine, One Baylor Plaza, 
      Houston, Texas, TX 77030, USA.
FAU - Chua, S S
AU  - Chua SS
FAU - DeMayo, F J
AU  - DeMayo FJ
FAU - Tsai, S Y
AU  - Tsai SY
LA  - eng
PT  - Journal Article
PT  - Research Support, U.S. Gov't, Non-P.H.S.
PL  - England
TA  - Oncogene
JT  - Oncogene
JID - 8711562
RN  - 0 (Cell Cycle Proteins)
RN  - EC 3.1.3.16 (Phosphoprotein Phosphatases)
RN  - EC 3.1.3.48 (CDC25B protein, human)
RN  - EC 3.1.3.48 (cdc25 Phosphatases)
SB  - IM
MH  - Animals
MH  - Cell Cycle Proteins/biosynthesis/genetics/*physiology
MH  - Cell Transformation, Neoplastic
MH  - Female
MH  - G1 Phase
MH  - Gene Expression
MH  - Genetic Vectors
MH  - Humans
MH  - Hyperplasia
MH  - Mammary Glands, Animal/*pathology
MH  - Mammary Tumor Virus, Mouse/genetics
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - Mice, Transgenic
MH  - Phosphoprotein Phosphatases/biosynthesis/genetics/*physiology
MH  - Promoter Regions, Genetic
MH  - Rabbits
MH  - S Phase
MH  - *cdc25 Phosphatases
EDAT- 1999/09/01 00:00
MHDA- 1999/09/01 00:01
CRDT- 1999/09/01 00:00
PHST- 1999/09/01 00:00 [pubmed]
PHST- 1999/09/01 00:01 [medline]
PHST- 1999/09/01 00:00 [entrez]
AID - 10.1038/sj.onc.1202809 [doi]
PST - ppublish
SO  - Oncogene. 1999 Aug 12;18(32):4564-76. doi: 10.1038/sj.onc.1202809.