PMID- 10469445
OWN - NLM
STAT- MEDLINE
DCOM- 19991014
LR  - 20221207
IS  - 0738-1085 (Print)
IS  - 0738-1085 (Linking)
VI  - 19
IP  - 6
DP  - 1999
TI  - Neovascularized bone grafts: experimental investigation.
PG  - 289-95
AB  - Vascularized bone grafts are standardized procedures in reconstructive surgery 
      but there are some disadvantages: donor site morbidity, limited number of 
      "natural" donor sites, and complex technique. In this study, we test the 
      possibility of creating a "neovascularized" bone graft utilizing a vascular 
      implantation procedure in a rabbit model. Sixteen New Zealand adult white rabbits 
      were used. In each animal, two iliac crest bone grafts (7 x 7 x 10 mm) were 
      harvested. Vascular implantation of the right superficial femoral vessels was 
      performed in one of the two grafts, which was wrapped in a silicone envelope to 
      avoid neovascularization from the surrounding tissues and positioned in a 
      subcutaneous pocket in the right medial thigh. On the left side, the bone block, 
      wrapped in the silicone envelope, was buried subcutaneously without vascular 
      implantation. The operated animals were divided into two groups: Group I included 
      eight rabbits explanted 4 weeks postoperatively and Group II included eight 
      rabbits explanted 8 weeks postoperatively. Tetracycline injection was performed 
      72 hours preexplantation to evaluate new bone formation. Selective colloidal ink 
      injection in the axial artery was performed to investigate the neovascularization 
      before inclusion in poly-methyl-methacrylate (PMMA). Histological examination was 
      performed in all explanted specimens comparatively. Histological examination 8 
      weeks after surgery showed a marked neovascularization, with normal bone cells. 
      Tetracycline labeling showed new bone formation with a normal pattern. In all 
      nonvascularized specimens, no viable cells or neovascularization and no bone 
      formation were found. The vascular implantation procedure can induce a good 
      neovascularization with new bone formation in a small bone graft. The possibility 
      of neovascularization induction by the simple vascular implantation procedure has 
      several clinical implications in reconstructive surgery.
CI  - Copyright 1999 Wiley-Liss, Inc.
FAU - Busa, R
AU  - Busa R
AD  - Unita Operativa di Chirurgia della Mano, University of Modena, Modena, Italy. 
      r.busa@mo.nettuno.it
FAU - Adani, R
AU  - Adani R
FAU - Castagnetti, C
AU  - Castagnetti C
FAU - Zaffe, D
AU  - Zaffe D
FAU - Mingione, A
AU  - Mingione A
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Microsurgery
JT  - Microsurgery
JID - 8309230
SB  - IM
MH  - Animals
MH  - Bone Development
MH  - Bone Transplantation/*methods
MH  - Bone and Bones/blood supply
MH  - Male
MH  - Rabbits
MH  - Plastic Surgery Procedures
MH  - Vascular Surgical Procedures/*methods
EDAT- 1999/09/01 00:00
MHDA- 1999/09/01 00:01
CRDT- 1999/09/01 00:00
PHST- 1999/09/01 00:00 [pubmed]
PHST- 1999/09/01 00:01 [medline]
PHST- 1999/09/01 00:00 [entrez]
AID - 10.1002/(SICI)1098-2752(1999)19:6<289::AID-MICR7>3.0.CO;2-# [pii]
AID - 10.1002/(sici)1098-2752(1999)19:6<289::aid-micr7>3.0.co;2-# [doi]
PST - ppublish
SO  - Microsurgery. 1999;19(6):289-95. doi: 
      10.1002/(sici)1098-2752(1999)19:6<289::aid-micr7>3.0.co;2-#.