PMID- 10470110 OWN - NLM STAT- MEDLINE DCOM- 19990920 LR - 20071114 IS - 0250-7005 (Print) IS - 0250-7005 (Linking) VI - 19 IP - 3A DP - 1999 May-Jun TI - Conventional cytogenetics alone is not sufficient for identifying interspecies cell line contamination. PG - 1753-4 AB - Evidence from our laboratory suggests that conventional chromosome banding analysis alone is not sufficient for detecting interspecies contamination of cell lines. To differentiate noncycling interphase cells of murine or other species origin that may be contaminating a human cell line, molecular cytogenetic techniques such as fluorescence in situ hybridization (FISH) with a total human DNA probe must be used. FAU - Multani, A S AU - Multani AS AD - Department of Cancer Biology, University of Texas M. D. Anderson Cancer Center Houston 77030, USA. FAU - Pathak, S AU - Pathak S LA - eng GR - RRO 4999-01/RR/NCRR NIH HHS/United States PT - Journal Article PT - Research Support, Non-U.S. Gov't PT - Research Support, U.S. Gov't, P.H.S. PL - Greece TA - Anticancer Res JT - Anticancer research JID - 8102988 RN - 0 (DNA Probes) SB - IM MH - Animal Population Groups/genetics MH - Animals MH - Bone Neoplasms/pathology MH - Cell Culture Techniques/*methods MH - Cell Cycle MH - *Cell Line MH - Chromosomes/genetics/ultrastructure MH - Chromosomes, Human/genetics/ultrastructure MH - Cricetinae MH - Cytogenetics/*methods MH - DNA Probes MH - Humans MH - *In Situ Hybridization, Fluorescence MH - Mice MH - Mice, Nude MH - Neoplasm Transplantation MH - Osteosarcoma/pathology MH - Species Specificity MH - Transplantation, Heterologous EDAT- 1999/09/02 00:00 MHDA- 1999/09/02 00:01 CRDT- 1999/09/02 00:00 PHST- 1999/09/02 00:00 [pubmed] PHST- 1999/09/02 00:01 [medline] PHST- 1999/09/02 00:00 [entrez] PST - ppublish SO - Anticancer Res. 1999 May-Jun;19(3A):1753-4.