PMID- 10471125
OWN - NLM
STAT- MEDLINE
DCOM- 20000124
LR  - 20190915
IS  - 0952-3278 (Print)
IS  - 0952-3278 (Linking)
VI  - 60
IP  - 5-6
DP  - 1999 May-Jun
TI  - Role of peroxisomal oxidation in the conversion of arachidonic acid to 
      eicosatrienoic acid in human skin fibroblasts.
PG  - 377-82
AB  - Human skin fibroblasts converted [5,6,8,9,11,12,14,15-3H]arachidonic acid 
      ([3H]20:4) to eicosatrienoic acid (20:3), but appreciable amounts of radiolabeled 
      20:3 were not detected in corresponding incubations with [1-(14)C]20:4. This 
      indicates that the main pathway for synthesizing 20:3 from arachidonic acid in 
      the fibroblast involves oxidative removal of the carboxyl group of arachidonic 
      acid. Fibroblasts deficient in long-chain acyl coenzyme A dehydrogenase (LCAD) 
      converted [3H]20:4 to [3H]20:3. However, Zellweger fibroblasts that are deficient 
      in peroxisomal fatty acid oxidation did not, indicating that the oxidative 
      removal of the carboxyl group occurs in the peroxisomes. [3H]Hexadecatrienoic 
      acid (16:3) was the main product that accumulated when [3H]20:4 was incubated 
      with normal, LCAD deficient, and very long-chain acyl coenzyme A dehydrogenase 
      (VLCAD) deficient fibroblasts, but Zellweger fibroblasts did not form this 
      product. Normal fibroblasts converted [3H]16:3 to radiolabeled 20:3 and 
      arachidonic acid. These findings suggest that some of the 16:3 produced from 
      arachidonic acid by peroxisomal beta-oxidation can be recycled and that this 
      recycling process constitutes a novel pathway for the conversion of arachidonic 
      acid to 20:3 in human fibroblasts.
FAU - Spector, A A
AU  - Spector AA
AD  - Department of Biochemistry, University of Iowa, Iowa City 52242, USA. 
      arthur-spector@uiowa.edu
FAU - Willard, D E
AU  - Willard DE
FAU - Kaduce, T L
AU  - Kaduce TL
FAU - Widstrom, R L
AU  - Widstrom RL
LA  - eng
GR  - CA 66081/CA/NCI NIH HHS/United States
GR  - HL49264/HL/NHLBI NIH HHS/United States
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - Scotland
TA  - Prostaglandins Leukot Essent Fatty Acids
JT  - Prostaglandins, leukotrienes, and essential fatty acids
JID - 8802730
RN  - 0 (Culture Media)
RN  - 0 (Fatty Acids)
RN  - 10028-17-8 (Tritium)
RN  - 27YG812J1I (Arachidonic Acid)
RN  - FC398RK06S (8,11,14-Eicosatrienoic Acid)
SB  - IM
MH  - 8,11,14-Eicosatrienoic Acid/*metabolism
MH  - Arachidonic Acid/*metabolism
MH  - Cell Line
MH  - Culture Media
MH  - Fatty Acids/analysis/metabolism
MH  - Fibroblasts/*metabolism/physiology
MH  - Humans
MH  - *Lipid Peroxidation
MH  - Peroxisomes/metabolism/*physiology
MH  - Skin/cytology/metabolism
MH  - Time Factors
MH  - Tritium/analysis/metabolism
EDAT- 1999/09/02 00:00
MHDA- 1999/09/02 00:01
CRDT- 1999/09/02 00:00
PHST- 1999/09/02 00:00 [pubmed]
PHST- 1999/09/02 00:01 [medline]
PHST- 1999/09/02 00:00 [entrez]
AID - 10.1016/s0952-3278(99)80016-7 [doi]
PST - ppublish
SO  - Prostaglandins Leukot Essent Fatty Acids. 1999 May-Jun;60(5-6):377-82. doi: 
      10.1016/s0952-3278(99)80016-7.