PMID- 10471661
OWN - NLM
STAT- MEDLINE
DCOM- 19990915
LR  - 20190722
IS  - 0009-9147 (Print)
IS  - 0009-9147 (Linking)
VI  - 45
IP  - 9
DP  - 1999 Sep
TI  - Evaluation of a particle-enhanced turbidimetric immunoassay for the measurement 
      of immunoglobulin E in an ILab 900 analyzer.
PG  - 1557-61
AB  - BACKGROUND: The measurement of immunoglobulin E (IgE) in serum is widely used in 
      the diagnosis of allergic reactions and parasitic infections. We describe here a 
      fully automated assay for human IgE suitable for routine application in a general 
      chemistry analyzer. METHODS: We used an ILab 900 analyzer. This instrument 
      automates a particle-enhanced immunoturbidimetric assay with an analysis time of 
      9 min. RESULTS: The assay was linear in the range 4-1000 kIU/L (r = 0.9998). The 
      intra- and interassay CVs at 57, 235, and 434 kIU/L were <3.5% and <7.4%, 
      respectively. The detection limit was 4 kIU/L. Hemoglobin (</=16 g/L), bilirubin 
      (</=250 micromol/L), and myeloma paraproteins did not interfere with the assay. 
      The assay showed good correlation with a microparticle enzyme immunoassay (r = 
      0.998) with a mean difference between methods of -6 +/- 26 kIU/L. CONCLUSION: The 
      new automated serum assay for IgE is an attractive alternative that avoids the 
      need for dedicated instrumentation.
FAU - Guaita, S
AU  - Guaita S
AD  - Centre de Recerca Biomedica, Hospital Universitari de Sant Joan, Carrer Sant Joan 
      s/n, 43201-Reus, Catalunya, Spain.
FAU - Simo, J M
AU  - Simo JM
FAU - Ferre, N
AU  - Ferre N
FAU - Joven, J
AU  - Joven J
FAU - Camps, J
AU  - Camps J
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Clin Chem
JT  - Clinical chemistry
JID - 9421549
RN  - 37341-29-0 (Immunoglobulin E)
SB  - IM
MH  - Autoanalysis
MH  - Humans
MH  - Immunoassay/methods
MH  - Immunoglobulin E/*blood
MH  - Nephelometry and Turbidimetry
MH  - Paraproteinemias/blood
EDAT- 1999/09/03 00:00
MHDA- 1999/09/03 00:01
CRDT- 1999/09/03 00:00
PHST- 1999/09/03 00:00 [pubmed]
PHST- 1999/09/03 00:01 [medline]
PHST- 1999/09/03 00:00 [entrez]
PST - ppublish
SO  - Clin Chem. 1999 Sep;45(9):1557-61.