PMID- 10473178
OWN - NLM
STAT- MEDLINE
DCOM- 19991209
LR  - 20190831
IS  - 0378-8741 (Print)
IS  - 0378-8741 (Linking)
VI  - 66
IP  - 3
DP  - 1999 Sep
TI  - Production of nitric oxide in mouse peritoneal macrophages after priming with 
      interferon-gamma by the stem of Sinomenium acutum.
PG  - 311-7
AB  - The present study demonstrates that the aqueous extract of Sinomenium acutum stem 
      (SSAE) produces nitric oxide (NO) upon treatment with recombinant interferon 
      gamma (rIFN-gamma) in mouse peritoneal macrophages. Apparently SSAE has no effect 
      on NO production by itself. This production is dependent on L-arginine and can be 
      inhibited by the L-arginine analogue N(G)-monomethyl-L-arginine. The increased 
      production of NO from rIFN-gamma plus SSAE-stimulated cells was decreased by the 
      treatment of protein kinase C inhibitor. Tumor necrosis factor-alpha (TNF-alpha) 
      has been shown to stimulate the oxidative metabolism of L-arginine to produce NO. 
      Mouse peritoneal macrophages secrete high levels of TNF-alpha after incubation 
      with rIFN-gamma plus SSAE. In addition, SSAE-induced NO production is 
      progressively inhibited by anti-murine TNF-alpha neutralizing antibody. These 
      results show that the capacity of SSAE to increase NO production from 
      rIFN-gamma-primed mouse peritoneal macrophages is the result of SSAE-induced 
      TNF-alpha secretion.
FAU - Kim, H M
AU  - Kim HM
AD  - Department of Oriental Pharmacy, College of Pharmacy, Wonkwang University, Iksan, 
      Chonbuk, South Korea. hmkim@med.wonkwang.ac.kr
FAU - Oh, D I
AU  - Oh DI
FAU - Chung, C K
AU  - Chung CK
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Ireland
TA  - J Ethnopharmacol
JT  - Journal of ethnopharmacology
JID - 7903310
RN  - 0 (Antibodies)
RN  - 0 (Nitrates)
RN  - 0 (Nitrites)
RN  - 0 (Plant Extracts)
RN  - 0 (Protein Kinase Inhibitors)
RN  - 0 (Recombinant Proteins)
RN  - 0 (Tumor Necrosis Factor-alpha)
RN  - 27JT06E6GR (omega-N-Methylarginine)
RN  - 31C4KY9ESH (Nitric Oxide)
RN  - 82115-62-6 (Interferon-gamma)
RN  - 94ZLA3W45F (Arginine)
SB  - IM
MH  - Animals
MH  - Antibodies/genetics
MH  - Arginine/pharmacology
MH  - Cells, Cultured
MH  - Interferon-gamma/*pharmacology
MH  - Macrophages, Peritoneal/*drug effects/metabolism
MH  - Mice
MH  - Neutralization Tests
MH  - Nitrates/analysis
MH  - Nitric Oxide/*biosynthesis
MH  - Nitrites/analysis
MH  - Plant Extracts/*pharmacology
MH  - *Protein Kinase Inhibitors
MH  - Recombinant Proteins
MH  - Tumor Necrosis Factor-alpha/antagonists & inhibitors/immunology
MH  - omega-N-Methylarginine/pharmacology
EDAT- 1999/09/03 00:00
MHDA- 1999/09/03 00:01
CRDT- 1999/09/03 00:00
PHST- 1999/09/03 00:00 [pubmed]
PHST- 1999/09/03 00:01 [medline]
PHST- 1999/09/03 00:00 [entrez]
AID - S0378874198002189 [pii]
AID - 10.1016/s0378-8741(98)00218-9 [doi]
PST - ppublish
SO  - J Ethnopharmacol. 1999 Sep;66(3):311-7. doi: 10.1016/s0378-8741(98)00218-9.