PMID- 10473419
OWN - NLM
STAT- MEDLINE
DCOM- 19991223
LR  - 20200724
IS  - 0099-2240 (Print)
IS  - 1098-5336 (Electronic)
IS  - 0099-2240 (Linking)
VI  - 65
IP  - 9
DP  - 1999 Sep
TI  - Identification of some of the major groups of bacteria in efficient and 
      nonefficient biological phosphorus removal activated sludge systems.
PG  - 4077-84
AB  - To investigate the bacteria that are important to phosphorus (P) removal in 
      activated sludge, microbial populations were analyzed during the operation of a 
      laboratory-scale reactor with various P removal performances. The bacterial 
      population structure, analyzed by fluorescence in situ hybridization (FISH) with 
      oligonucleotides probes complementary to regions of the 16S and 23S rRNAs, was 
      associated with the P removal performance of the reactor. At one stage of the 
      reactor operation, chemical characterization revealed that extremely poor P 
      removal was occurring. However, like in typical P-removing sludges, complete 
      anaerobic uptake of the carbon substrate occurred. Bacteria inhibiting P removal 
      overwhelmed the reactor, and according to FISH, bacteria of the beta subclass of 
      the class Proteobacteria other than beta-1 or beta-2 were dominant in the sludge 
      (58% of the population). Changes made to the operation of the reactor led to the 
      development of a biomass population with an extremely good P removal capacity. 
      The biochemical transformations observed in this sludge were characteristic of 
      typical P-removing activated sludge. The microbial population analysis of the 
      P-removing sludge indicated that bacteria of the beta-2 subclass of the class 
      Proteobacteria and actinobacteria were dominant (55 and 35%, respectively), 
      therefore implicating bacteria from these groups in high-performance P removal. 
      The changes in operation that led to the improved performance of the reactor 
      included allowing the pH to rise during the anaerobic period, which promoted 
      anaerobic phosphate release and possibly caused selection against 
      non-phosphate-removing bacteria.
FAU - Bond, P L
AU  - Bond PL
AD  - Advanced Wastewater Management Centre, Departments of Chemical Engineering and 
      Microbiology and Parasitology, The University of Queensland, Brisbane, 
      Queensland, 4072, Australia.
FAU - Erhart, R
AU  - Erhart R
FAU - Wagner, M
AU  - Wagner M
FAU - Keller, J
AU  - Keller J
FAU - Blackall, L L
AU  - Blackall LL
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Appl Environ Microbiol
JT  - Applied and environmental microbiology
JID - 7605801
RN  - 0 (Oligonucleotide Probes)
RN  - 0 (RNA, Bacterial)
RN  - 0 (RNA, Ribosomal, 16S)
RN  - 0 (RNA, Ribosomal, 23S)
RN  - 0 (Sewage)
RN  - 27YLU75U4W (Phosphorus)
SB  - IM
MH  - Bacteria/*classification/genetics/growth & development/*metabolism
MH  - Bioreactors
MH  - Ecosystem
MH  - In Situ Hybridization, Fluorescence
MH  - Oligonucleotide Probes
MH  - Phosphorus/*metabolism
MH  - RNA, Bacterial/genetics
MH  - RNA, Ribosomal, 16S/genetics
MH  - RNA, Ribosomal, 23S/genetics
MH  - Sewage/*microbiology
PMC - PMC99744
EDAT- 1999/09/03 00:00
MHDA- 1999/09/03 00:01
CRDT- 1999/09/03 00:00
PHST- 1999/09/03 00:00 [pubmed]
PHST- 1999/09/03 00:01 [medline]
PHST- 1999/09/03 00:00 [entrez]
AID - 0102 [pii]
AID - 10.1128/AEM.65.9.4077-4084.1999 [doi]
PST - ppublish
SO  - Appl Environ Microbiol. 1999 Sep;65(9):4077-84. doi: 
      10.1128/AEM.65.9.4077-4084.1999.