PMID- 10474198
OWN - NLM
STAT- MEDLINE
DCOM- 19991018
LR  - 20181130
IS  - 0378-1097 (Print)
IS  - 0378-1097 (Linking)
VI  - 177
IP  - 2
DP  - 1999 Aug 15
TI  - Adherence and internalization of Streptococcus uberis to bovine mammary 
      epithelial cells are mediated by host cell proteoglycans.
PG  - 313-7
AB  - The role of cell glycosaminoglycans (GAG) on adherence and internalization of 
      Streptococcus uberis to bovine mammary epithelial cells was evaluated by 
      adherence/internalization competition assays, by removal of GAG from the host 
      cell surface and by inhibition of GAG glycosylation in the host cell. Heparin 
      (HEP), heparan sulfate (HSA), chondroitin sulfate A (CSA) and chondroitin sulfate 
      B (CSB) inhibited adherence and internalization of S. uberis in a dose-dependent 
      manner. However, inhibition was lower with CSA and CSB than that observed with 
      HEP and HSA. Adherence and internalization were also inhibited upon treatment of 
      mammary epithelial cells with GAG lyases. The greatest inhibition was observed 
      with heparinase I. Tunicamycin, an inhibitor of mammalian cell glycosylation of 
      cell surface glycoproteins, markedly inhibited internalization of S. uberis into 
      mammary epithelial cells. Differences between strains were observed. These 
      results suggest that a HSA proteoglycan receptor on the host cell surface may 
      mediate S. uberis adherence to and internalization of bovine mammary epithelial 
      cells.
FAU - Almeida, R A
AU  - Almeida RA
AD  - Department of Animal Science, University of Tennessee, Knoxville 37996, USA.
FAU - Fang, W
AU  - Fang W
FAU - Oliver, S P
AU  - Oliver SP
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, Non-P.H.S.
PL  - England
TA  - FEMS Microbiol Lett
JT  - FEMS microbiology letters
JID - 7705721
RN  - 0 (Glycosaminoglycans)
SB  - IM
MH  - Animals
MH  - Bacterial Adhesion/*drug effects
MH  - Cattle
MH  - Epithelial Cells/*microbiology
MH  - Female
MH  - Glycosaminoglycans/*pharmacology
MH  - Mammary Glands, Animal/*microbiology
MH  - Mastitis, Bovine/etiology
MH  - Streptococcus/*pathogenicity
EDAT- 1999/09/04 00:00
MHDA- 1999/09/04 00:01
CRDT- 1999/09/04 00:00
PHST- 1999/09/04 00:00 [pubmed]
PHST- 1999/09/04 00:01 [medline]
PHST- 1999/09/04 00:00 [entrez]
AID - S0378-1097(99)00332-8 [pii]
AID - 10.1111/j.1574-6968.1999.tb13748.x [doi]
PST - ppublish
SO  - FEMS Microbiol Lett. 1999 Aug 15;177(2):313-7. doi: 
      10.1111/j.1574-6968.1999.tb13748.x.