PMID- 10474821
OWN - NLM
STAT- MEDLINE
DCOM- 19991008
LR  - 20190513
IS  - 0267-8357 (Print)
IS  - 0267-8357 (Linking)
VI  - 14
IP  - 1
DP  - 1999 Jan
TI  - Noscapine hydrochloride disrupts the mitotic spindle in mammalian cells and 
      induces aneuploidy as well as polyploidy in cultured human lymphocytes.
PG  - 51-6
AB  - Noscapine hydrochloride is a centrally acting antitussive opium derivative widely 
      used in cough suppressants. Recent studies have reported that noscapine is a 
      potent inducer of polyploidy but not of aneuploidy in vitro. To obtain more 
      comprehensive information about the cytogenetic effects of this compound, we 
      treated cultured human lymphocytes (HPL) and Chinese hamster ovary (CHO) cells 
      with various concentrations of noscapine hydrochloride. Using a differential 
      staining technique noscapine was shown to disrupt the mitotic spindle at 
      concentrations < 5 micrograms/ml in both cell types. The use of multicolor 
      fluorescence in situ hybridization (FISH) on noscapine-treated human lymphocytes 
      showed a dose-dependent induction of hyperdiploidy of chromosome 1 but not of 
      chromosomal breakage in the 1cen-q12 region under in vitro conditions, indicating 
      that noscapine is specifically inducing numerical chromosomal aberrations. FISH 
      with probes targeting different chromosomes revealed that noscapine is capable of 
      inducing both polyploidy and true hyperdiploidy. Our results show that noscapine, 
      by disrupting the function of the mitotic spindle, has the ability to induce 
      aneuploidy and not uniquely polyploidy as previously reported. By using these 
      types of molecular cytogenetic techniques, it should be possible to evaluate the 
      ability of noscapine to induce aneuploidy in the upper intestinal tract in vivo.
FAU - Schuler, M
AU  - Schuler M
AD  - Environmental Toxicology Graduate Program, University of California, Riverside 
      92521, USA.
FAU - Muehlbauer, P
AU  - Muehlbauer P
FAU - Guzzie, P
AU  - Guzzie P
FAU - Eastmond, D A
AU  - Eastmond DA
LA  - eng
PT  - Journal Article
PL  - England
TA  - Mutagenesis
JT  - Mutagenesis
JID - 8707812
RN  - 8V32U4AOQU (Noscapine)
SB  - IM
MH  - *Aneuploidy
MH  - Animals
MH  - CHO Cells
MH  - Cells, Cultured
MH  - Cricetinae
MH  - Dose-Response Relationship, Drug
MH  - Humans
MH  - In Situ Hybridization, Fluorescence/methods
MH  - Lymphocytes/*drug effects
MH  - Mutagenesis
MH  - Noscapine/*toxicity
MH  - *Polyploidy
MH  - Spindle Apparatus/*drug effects
EDAT- 1999/09/04 00:00
MHDA- 1999/09/04 00:01
CRDT- 1999/09/04 00:00
PHST- 1999/09/04 00:00 [pubmed]
PHST- 1999/09/04 00:01 [medline]
PHST- 1999/09/04 00:00 [entrez]
AID - 10.1093/mutage/14.1.51 [doi]
PST - ppublish
SO  - Mutagenesis. 1999 Jan;14(1):51-6. doi: 10.1093/mutage/14.1.51.