PMID- 10475619
OWN - NLM
STAT- MEDLINE
DCOM- 19990923
LR  - 20190826
IS  - 0145-2126 (Print)
IS  - 0145-2126 (Linking)
VI  - 23
IP  - 9
DP  - 1999 Sep
TI  - A colorimetric assay to evaluate the chemotherapeutic response of children with 
      acute lymphoblastic leukemia (ALL) employing achatininH: a 9-O-acetyl sialic acid 
      binding lectin.
PG  - 803-9
AB  - Employing a 9-O-acetyl sialic acid binding lectin, Achatinin(H) (ATNH), we have 
      reported a non-invasive, blood based lymphoproliferation assay which measures the 
      maximal lymphoproliferative dose (MLD) of ATN(H) to assess the status of 
      9-O-acetylated sialoglycoconjugates (9-OAcSGs) in patients with Acute 
      lymphoblastic leukemia (ALL) (Mandal C, Sinha D, Sharma V, Bhattacharya DK. 
      O-acetyl sialic acid binding lectin, as a probe for detection of subtle changes 
      on the cell surface induced during acute lymphoblastic leukemia [ALL] and its 
      clinical application. Ind J Biochem Biophys 1997;34:82; Sinha D, Mandal C, 
      Bhattacharya DK. Development of a simple blood based lymphoproliferation assay to 
      assess the clinical status of patients with acute lymphoblastic leukemia. Leuk 
      Res 1999;13:309-312; Sinha D, Mandal C, Bhattacharya DK. A novel method for 
      prognostic evaluation of childhood acute lymphoblastic leukemia. Leukemia 
      1999;13[in press]). Although the expression of 9-OAcSGs clearly serves as an 
      index of treatment outcome, the assay has limitations in that it requires 
      radioisotopes, i.e. [3H]-TdR. Therefore a colorimetric assay was developed as an 
      alternative approach. The pre-treatment MLD, as measured by the colorimetric 
      assay, was 0.15 +/- 0.02 microg which progressively increased during 
      consolidation therapy (1.40 +/- 0.39 microg), maintenance therapy (4.20 +/- 1.60 
      microg) and in followed-up cases (5.20 +/- 0.43 microg) but sharply declined 
      following relapse (0.25 +/- 0.02 microg). The colorimetric assay also showed a 
      good correlation with radiometric assay (r = + 0.93) and their mean coefficient 
      of inter-assay precision were also comparable (15.53% versus 14.86%). We 
      therefore propose that the colorimetric assay is a safe, non-radiometric, 
      user-friendly alternative for assessing individual chemotherapeutic responses in 
      childhood ALL.
FAU - Sinha, D
AU  - Sinha D
AD  - Department of Immunobiology, Indian Institute of Chemical Biology, Calcutta.
FAU - Bhattacharya, D K
AU  - Bhattacharya DK
FAU - Mandal, C
AU  - Mandal C
LA  - eng
PT  - Clinical Trial
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Leuk Res
JT  - Leukemia research
JID - 7706787
RN  - 0 (Coloring Agents)
RN  - 0 (Lectins)
RN  - 0 (Tetrazolium Salts)
RN  - 0 (Thiazoles)
RN  - 0 (achatinin(H))
RN  - EUY85H477I (thiazolyl blue)
RN  - GZP2782OP0 (N-Acetylneuraminic Acid)
SB  - IM
MH  - Acetylation
MH  - Child
MH  - Child, Preschool
MH  - Colorimetry
MH  - Coloring Agents
MH  - Evaluation Studies as Topic
MH  - Female
MH  - Humans
MH  - *Lectins
MH  - Lymphoproliferative Disorders/diagnosis
MH  - Male
MH  - N-Acetylneuraminic Acid/metabolism
MH  - Precursor Cell Lymphoblastic Leukemia-Lymphoma/*drug therapy
MH  - Tetrazolium Salts
MH  - Thiazoles
MH  - Treatment Outcome
EDAT- 1999/09/04 00:00
MHDA- 1999/09/04 00:01
CRDT- 1999/09/04 00:00
PHST- 1999/09/04 00:00 [pubmed]
PHST- 1999/09/04 00:01 [medline]
PHST- 1999/09/04 00:00 [entrez]
AID - S0145-2126(99)00093-4 [pii]
AID - 10.1016/s0145-2126(99)00093-4 [doi]
PST - ppublish
SO  - Leuk Res. 1999 Sep;23(9):803-9. doi: 10.1016/s0145-2126(99)00093-4.