PMID- 10477021
OWN - NLM
STAT- MEDLINE
DCOM- 19990920
LR  - 20190708
IS  - 0360-3016 (Print)
IS  - 0360-3016 (Linking)
VI  - 45
IP  - 1
DP  - 1999 Aug 1
TI  - Oxygen tension measurements of tumors growing in mice.
PG  - 171-80
AB  - PURPOSE: Clinical studies using the Eppendorf histograph have shown that patients 
      whose tumors have a low pO2 have worse local control after radiotherapy, and have 
      higher metastatic rates. Because preclinical studies of methods of overcoming, or 
      exploiting, hypoxia generally use transplanted tumors in mice, we have compared 
      the oxygenation of mouse tumors with human tumors to determine the 
      appropriateness of the transplanted mouse model for such preclinical studies. 
      METHODS AND MATERIALS: We evaluated the oxygenation status of subcutaneous (s.c.) 
      tissue and of 12 intradermally (i.d.)- and 7 s.c.-growing mouse or human 
      transplanted tumors in mice using the Eppendorf histograph, and compared the 
      values obtained with measurements of human head and neck nodes. RESULTS: The 
      normal tissue pO2 profile of air-breathing mice showed a nearly Gaussian 
      distribution (38.2+/-14.9 mmHg). Breathing 10% O2 or carbogen resulted in 
      dramatic changes in normal tissue oxygenation. Tumors growing intradermally in 
      the back of air-breathing mice were extremely hypoxic and resistant to expected 
      changes in oxygenation (carbogen breathing, size, and use of anesthetics). Tumors 
      growing s.c. in the foot showed higher oxygen profiles with marked changes in 
      oxygenation when exposing the animals to different levels of oxygen. However, the 
      oxygenation of the mouse tumors transplanted in either site was only a fraction 
      of that of the majority of human tumors. CONCLUSION: Experimental mouse tumors 
      are markedly hypoxic, with median values of 10-20% of those of human tumors. 
      Hence, mouse tumors are probably good models for the most hypoxic human tumors 
      that respond poorly to radiotherapy; however, caution has to be exercised in 
      extrapolating data from mouse to man.
FAU - Adam, M F
AU  - Adam MF
AD  - Department of Radiation Oncology, Stanford University School of Medicine, CA 
      94305-5468, USA.
FAU - Dorie, M J
AU  - Dorie MJ
FAU - Brown, J M
AU  - Brown JM
LA  - eng
GR  - CA 67166/CA/NCI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Int J Radiat Oncol Biol Phys
JT  - International journal of radiation oncology, biology, physics
JID - 7603616
SB  - IM
MH  - Animals
MH  - Female
MH  - Humans
MH  - Ion-Selective Electrodes
MH  - Mice
MH  - Mice, Inbred C3H
MH  - Mice, SCID
MH  - Neoplasm Transplantation
MH  - Neoplasms/*physiopathology
MH  - *Oxygen Consumption
MH  - Partial Pressure
MH  - Radiobiology
MH  - Sensitivity and Specificity
MH  - Transplantation, Heterologous
MH  - Tumor Cells, Cultured
EDAT- 1999/09/07 00:00
MHDA- 1999/09/07 00:01
CRDT- 1999/09/07 00:00
PHST- 1999/09/07 00:00 [pubmed]
PHST- 1999/09/07 00:01 [medline]
PHST- 1999/09/07 00:00 [entrez]
AID - S0360301699001571 [pii]
AID - 10.1016/s0360-3016(99)00157-1 [doi]
PST - ppublish
SO  - Int J Radiat Oncol Biol Phys. 1999 Aug 1;45(1):171-80. doi: 
      10.1016/s0360-3016(99)00157-1.