PMID- 10477087
OWN - NLM
STAT- MEDLINE
DCOM- 19991216
LR  - 20190818
IS  - 0196-9781 (Print)
IS  - 0196-9781 (Linking)
VI  - 20
IP  - 7
DP  - 1999
TI  - Thrombin-stimulated increases in cytosolic Ca2+ level and gonadotropin-releasing 
      hormone release in GT1-7 neurons.
PG  - 859-64
AB  - The effects of thrombin on cytosolic calcium levels ([Ca2+]cyt), and on 
      gonadotropin-releasing hormone (GnRH) release, were characterized in cultured 
      GT1-7 neurons. GnRH release from GT1-7 neurons was pulsatile with an average 
      pulse amplitude of 14.3+/-5.8 pg x min x ml(-1) and an average pulse duration of 
      21.3+/-4.2 min. The [Ca2+]cyt response to 0.005 to 0.2 U/ml thrombin was 
      saturable and concentration dependent (EC50 = 0.0268 U/ml). 
      Ethyleneglycotetraacetic acid (EGTA) chelation of extracellular Ca2+ resulted in 
      an approximately 70% attenuation of thrombin-stimulated increase in [Ca2+]cyt. By 
      use of a special superfusion system, a 5-min exposure to 0.1 U/ml thrombin 
      significantly increased the amplitude (193.2+/-67.8 pg x min x ml(-1); P = 0.001) 
      but not the duration (22.5+/-2.4 min; P = 0.8) of GnRH release. These results 
      suggest that thrombin increases [Ca2+]cyt and GnRH release from GT1-7 neurons via 
      specific membrane-bound receptors.
FAU - Chen, E C
AU  - Chen EC
AD  - Department of Obstetrics and Gynecology, The University of Texas Health Science 
      Center, San Antonio 78284, USA. chene@uthscsa.edu
FAU - King, T S
AU  - King TS
FAU - Chang, X
AU  - Chang X
FAU - Norris, C
AU  - Norris C
FAU - Schenken, R S
AU  - Schenken RS
FAU - Javors, M A
AU  - Javors MA
LA  - eng
GR  - 10112/PHS HHS/United States
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Peptides
JT  - Peptides
JID - 8008690
RN  - 0 (Chelating Agents)
RN  - 27YG812J1I (Arachidonic Acid)
RN  - 33515-09-2 (Gonadotropin-Releasing Hormone)
RN  - 526U7A2651 (Egtazic Acid)
RN  - EC 3.1.1.32 (Phospholipases A)
RN  - EC 3.4.21.5 (Thrombin)
RN  - SY7Q814VUP (Calcium)
SB  - IM
MH  - Animals
MH  - Arachidonic Acid/metabolism
MH  - Calcium/*metabolism
MH  - Cell Line
MH  - Chelating Agents/pharmacology
MH  - Cytosol/metabolism
MH  - Egtazic Acid/pharmacology
MH  - Gonadotropin-Releasing Hormone/*metabolism
MH  - Mice
MH  - Neurons/*drug effects/*physiology
MH  - Phospholipases A/metabolism
MH  - Thrombin/*pharmacology
EDAT- 1999/09/07 00:00
MHDA- 1999/09/07 00:01
CRDT- 1999/09/07 00:00
PHST- 1999/09/07 00:00 [pubmed]
PHST- 1999/09/07 00:01 [medline]
PHST- 1999/09/07 00:00 [entrez]
AID - S0196-9781(99)00073-X [pii]
AID - 10.1016/s0196-9781(99)00073-x [doi]
PST - ppublish
SO  - Peptides. 1999;20(7):859-64. doi: 10.1016/s0196-9781(99)00073-x.