PMID- 10477349
OWN - NLM
STAT- MEDLINE
DCOM- 19990922
LR  - 20190702
IS  - 0027-5107 (Print)
IS  - 0027-5107 (Linking)
VI  - 444
IP  - 1
DP  - 1999 Jul 21
TI  - Cell cycle specificity of cytogenetic damage induced by 3,4-epoxy-1- butene.
PG  - 151-8
AB  - 3,4-epoxy-1-butene (EB), a primary metabolite of butadiene, is a direct-acting 
      "S-dependent" genotoxicant that can induce sister chromatid exchanges (SCEs) and 
      chromosome aberrations (CAs) in cycling cells in vitro. However, EB is almost 
      inactive when splenic or peripheral blood lymphocytes are exposed at the G(0) 
      stage of the cell cycle. To investigate whether repair of DNA lesions is 
      responsible for the lack of cytogenetic responses seen after G(0) treatments, we 
      used cytosine arabinoside (ara-C) to inhibit DNA polymerization during DNA 
      repair. If enough repairable lesions are present, double-strand breaks should 
      accumulate and form chromosome-type ("S-independent") deletions and exchanges. 
      This is exactly what occurred. EB induced chromosome deletions and dicentrics at 
      the first division following treatment, when the EB exposure was followed by 
      ara-C. Without ara-C treatment, there was no induction of CAs. These experiments 
      indicate that the relatively low levels of damage induced by EB in G(0) 
      lymphocytes are removed by DNA repair prior to DNA synthesis and thus, before the 
      production of SCEs or chromatid-type aberrations.
FAU - Kligerman, A D
AU  - Kligerman AD
AD  - Genetics and Cellular Toxicology Branch, Mail Drop 68, Environmental 
      Carcinogenesis Division, U.S. Environmental Protection Agency, Research Triangle 
      Park, NC 27711, USA. kligerman.andrew@epa.gov
FAU - Doerr, C L
AU  - Doerr CL
FAU - Tennant, A H
AU  - Tennant AH
LA  - eng
PT  - Journal Article
PL  - Netherlands
TA  - Mutat Res
JT  - Mutation research
JID - 0400763
RN  - 0 (Epoxy Compounds)
RN  - 0 (Mutagens)
RN  - 04079A1RDZ (Cytarabine)
RN  - 478ERR5NKR (3,4-epoxy-1-butene)
RN  - 9007-49-2 (DNA)
SB  - IM
MH  - Cell Cycle
MH  - Chromosome Aberrations
MH  - Cytarabine/pharmacology
MH  - DNA/biosynthesis/drug effects/genetics
MH  - DNA Repair/drug effects
MH  - Epoxy Compounds/*toxicity
MH  - Humans
MH  - In Vitro Techniques
MH  - Lymphocytes/cytology/drug effects/metabolism
MH  - Mutagens/*toxicity
MH  - Resting Phase, Cell Cycle
MH  - Sister Chromatid Exchange/drug effects
EDAT- 1999/09/08 00:00
MHDA- 1999/09/08 00:01
CRDT- 1999/09/08 00:00
PHST- 1999/09/08 00:00 [pubmed]
PHST- 1999/09/08 00:01 [medline]
PHST- 1999/09/08 00:00 [entrez]
AID - S1383571899000777 [pii]
AID - 10.1016/s1383-5718(99)00077-7 [doi]
PST - ppublish
SO  - Mutat Res. 1999 Jul 21;444(1):151-8. doi: 10.1016/s1383-5718(99)00077-7.