PMID- 10477592
OWN - NLM
STAT- MEDLINE
DCOM- 19991004
LR  - 20220408
IS  - 0022-1767 (Print)
IS  - 0022-1767 (Linking)
VI  - 163
IP  - 6
DP  - 1999 Sep 15
TI  - A role for IL-16 in the cross-talk between dendritic cells and T cells.
PG  - 3232-8
AB  - Dendritic cells (DCs) in the periphery capture and process Ags, migrate to 
      lymphoid organs, and initiate immune responses in T cells. IL-16, the soluble 
      ligand for CD4, is a potent chemoattractant for CD4+ T cells, eosinophils, and 
      monocytes and is mainly derived from activated T cells. Because migration is a 
      fundamental property of DCs, we asked whether IL-16 induces chemotaxis in DCs and 
      whether DCs are a source of IL-16. DCs were generated by culture of monocytes in 
      IL-4 and GM-CSF for 6 days and subsequently highly purified employing magnetic 
      beads. Migration was assayed by nitrocellulose and polycarbonate filter-based 
      assays, and distinction of chemotaxis and chemokinesis was performed by a 
      checkerboard analysis. Messenger RNA and protein data revealed constitutive 
      expression and release of IL-16 by day-6 DCs. Gradients of rIL-16 induced a 
      chemotactic response of DCs. Furthermore, the chemotactic activity of DC 
      supernatant toward DCs themselves and T cells was mainly due to IL-16, because 
      the addition of neutralizing Abs completely abrogated the migratory response. 
      However, after induction of maturation by the addition of TNF-alpha and PGE2 DCs, 
      neither expressed IL-16 mRNA nor produced IL-16 protein. We conclude that IL-16 
      may play a role in the trafficking of DCs and may be a major chemotactic signal 
      from DCs toward themselves and toward T cells.
FAU - Kaser, A
AU  - Kaser A
AD  - Division of Gastroenterology, Department of Medicine, University Hospital 
      Innsbruck, Austria.
FAU - Dunzendorfer, S
AU  - Dunzendorfer S
FAU - Offner, F A
AU  - Offner FA
FAU - Ryan, T
AU  - Ryan T
FAU - Schwabegger, A
AU  - Schwabegger A
FAU - Cruikshank, W W
AU  - Cruikshank WW
FAU - Wiedermann, C J
AU  - Wiedermann CJ
FAU - Tilg, H
AU  - Tilg H
LA  - eng
GR  - AI35680/AI/NIAID NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - J Immunol
JT  - Journal of immunology (Baltimore, Md. : 1950)
JID - 2985117R
RN  - 0 (Chemotactic Factors)
RN  - 0 (Interleukin-16)
RN  - 42HK56048U (Tyrosine)
SB  - IM
MH  - Cell Communication/*immunology
MH  - Cell-Free System/immunology
MH  - Cells, Cultured
MH  - Chemotactic Factors/physiology
MH  - Dendritic Cells/cytology/metabolism/*physiology
MH  - Epidermis/metabolism
MH  - Humans
MH  - Interleukin-16/biosynthesis/*physiology
MH  - Monocytes/immunology/metabolism
MH  - Phosphorylation
MH  - T-Lymphocytes/cytology/*physiology
MH  - Time Factors
MH  - Tyrosine/metabolism
EDAT- 1999/09/08 00:00
MHDA- 1999/09/08 00:01
CRDT- 1999/09/08 00:00
PHST- 1999/09/08 00:00 [pubmed]
PHST- 1999/09/08 00:01 [medline]
PHST- 1999/09/08 00:00 [entrez]
AID - ji_v163n6p3232 [pii]
PST - ppublish
SO  - J Immunol. 1999 Sep 15;163(6):3232-8.