PMID- 10477708
OWN - NLM
STAT- MEDLINE
DCOM- 19990929
LR  - 20210216
IS  - 0006-4971 (Print)
IS  - 0006-4971 (Linking)
VI  - 94
IP  - 5
DP  - 1999 Sep 1
TI  - Detection of abnormal pretransplant clones in progenitor cells of patients who 
      developed myelodysplasia after autologous transplantation.
PG  - 1814-9
AB  - Secondary myelodysplastic syndromes (MDS) have been reported after autologous 
      transplantation. It is not known whether the MDS results from the pretransplant 
      conventional-dose chemotherapy or from the high-dose chemotherapy (HDC) used for 
      the transplant procedure. We performed a multicenter, retrospective analysis of 
      morphologically normal pretransplant marrow or stem cell specimens from 12 
      patients who subsequently developed myelodysplasia after HDC. To determine if the 
      abnormal clone was present before HDC, we used fluorescence in situ hybridization 
      (FISH) to detect the cytogenetic markers observed at the onset of posttransplant 
      MDS. Cryopreserved, pretransplant bone marrow, peripheral blood stem cell 
      specimens, obtained at the time of harvest, or archival smears were used. 
      Standard cytogenetic analysis had been performed pretransplant in four patients, 
      showing a normal karyotype. In 9 of 12 cases, the same cytogenetic abnormality 
      observed at the time of MDS diagnosis was detected by FISH in the pre-HDC 
      specimens. Our findings support the hypothesis that, in many cases of 
      posttransplant MDS, the stem cell damage results from prior conventional-dose 
      chemotherapy and may be unrelated to HDC or the transplantation process itself.
FAU - Abruzzese, E
AU  - Abruzzese E
AD  - Section on Hematology and Oncology, the Department of Medicine, Wake Forest 
      University School of Medicine, Winston-Salem, NC 27157-1082, USA.
FAU - Radford, J E
AU  - Radford JE
FAU - Miller, J S
AU  - Miller JS
FAU - Vredenburgh, J J
AU  - Vredenburgh JJ
FAU - Rao, P N
AU  - Rao PN
FAU - Pettenati, M J
AU  - Pettenati MJ
FAU - Cruz, J M
AU  - Cruz JM
FAU - Perry, J J
AU  - Perry JJ
FAU - Amadori, S
AU  - Amadori S
FAU - Hurd, D D
AU  - Hurd DD
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Blood
JT  - Blood
JID - 7603509
SB  - IM
MH  - Adult
MH  - Bone Marrow Transplantation/*adverse effects
MH  - Female
MH  - Hematopoietic Stem Cell Mobilization/adverse effects
MH  - Hematopoietic Stem Cells/*pathology
MH  - Humans
MH  - Karyotyping
MH  - Male
MH  - Middle Aged
MH  - Myelodysplastic Syndromes/*etiology/*pathology
MH  - Neoplasms/*pathology/therapy
MH  - Transplantation, Autologous
EDAT- 1999/09/09 00:00
MHDA- 1999/09/09 00:01
CRDT- 1999/09/09 00:00
PHST- 1999/09/09 00:00 [pubmed]
PHST- 1999/09/09 00:01 [medline]
PHST- 1999/09/09 00:00 [entrez]
AID - S0006-4971(20)48880-9 [pii]
PST - ppublish
SO  - Blood. 1999 Sep 1;94(5):1814-9.