PMID- 10477734
OWN - NLM
STAT- MEDLINE
DCOM- 19991012
LR  - 20210216
IS  - 0006-4971 (Print)
IS  - 0006-4971 (Linking)
VI  - 94
IP  - 6
DP  - 1999 Sep 15
TI  - CD34(+) acute myeloid and lymphoid leukemic blasts can be induced to 
      differentiate into dendritic cells.
PG  - 2048-55
AB  - CD34(+) hematopoietic stem cells from normal individuals and from patients with 
      chronic myelogenous leukemia can be induced to differentiate into dendritic cells 
      (DC). The aim of the current study was to determine whether acute myeloid 
      leukemia (AML) and acute lymphoblastic leukemia (ALL) cells could be induced to 
      differentiate into DC. CD34(+) AML-M2 cells with chromosome 7 monosomy were 
      cultured in the presence of granulocyte-macrophage colony-stimulating factor 
      (GM-CSF), tumor necrosis factor alpha (TNFalpha), and interleukin-4 (IL-4). After 
      3 weeks of culture, 35% of the AML-M2 cells showed DC morphology and phenotype. 
      The DC phenotype was defined as upmodulation of the costimulatory molecules CD80 
      and CD86 and the expression of CD1a or CD83. The leukemic nature of the DC was 
      validated by detection of chromosome 7 monosomy in sorted DC populations by 
      fluorescence in situ hybridization (FISH). CD34(+) leukemic cells from 2 B-ALL 
      patients with the Philadelphia chromosome were similarly cultured, but in the 
      presence of CD40-ligand and IL-4. After 4 days of culture, more than 58% of the 
      ALL cells showed DC morphology and phenotype. The leukemic nature of the DC was 
      validated by detection of the bcr-abl fusion gene in sorted DC populations by 
      FISH. In functional studies, the leukemic DC were highly superior to the parental 
      leukemic blasts for inducing allogeneic T-cell responses. Thus, CD34(+) AML and 
      ALL cells can be induced to differentiate into leukemic DC with morphologic, 
      phenotypic, and functional similarities to normal DC.
FAU - Cignetti, A
AU  - Cignetti A
AD  - Corixa Corp, Seattle, WA; the Fred Hutchinson Cancer Research Center, Seattle, 
      WA; the Divisione Ospedaliera di Ematologia, Azienda Ospedaliera S. Giovanni 
      Battista, Torino, Italy.
FAU - Bryant, E
AU  - Bryant E
FAU - Allione, B
AU  - Allione B
FAU - Vitale, A
AU  - Vitale A
FAU - Foa, R
AU  - Foa R
FAU - Cheever, M A
AU  - Cheever MA
LA  - eng
GR  - 5 R37 CA30558/CA/NCI NIH HHS/United States
GR  - CA81029/CA/NCI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Blood
JT  - Blood
JID - 7603509
RN  - 0 (Antigens, CD)
RN  - 0 (Antigens, CD34)
SB  - IM
MH  - Antigens, CD/blood
MH  - Antigens, CD34/blood
MH  - Cell Differentiation
MH  - Cells, Cultured
MH  - Chromosomes, Human, Pair 7
MH  - Dendritic Cells/immunology/pathology
MH  - Flow Cytometry
MH  - Genotype
MH  - Hematopoietic Stem Cells/*immunology/pathology
MH  - Humans
MH  - Immunophenotyping
MH  - Leukemia, Myeloid, Acute/*blood/genetics/immunology/pathology
MH  - Monosomy
MH  - Precursor Cell Lymphoblastic Leukemia-Lymphoma/*blood/immunology/pathology
MH  - Time Factors
EDAT- 1999/09/09 00:00
MHDA- 1999/09/09 00:01
CRDT- 1999/09/09 00:00
PHST- 1999/09/09 00:00 [pubmed]
PHST- 1999/09/09 00:01 [medline]
PHST- 1999/09/09 00:00 [entrez]
AID - S0006-4971(20)48912-8 [pii]
PST - ppublish
SO  - Blood. 1999 Sep 15;94(6):2048-55.