PMID- 10478804
OWN - NLM
STAT- MEDLINE
DCOM- 19990921
LR  - 20181113
IS  - 1071-2690 (Print)
IS  - 1071-2690 (Linking)
VI  - 35
IP  - 4
DP  - 1999 Apr
TI  - Amplification of the Y chromosome in three murine tumor cell lines transformed in 
      vivo by different human prostate cancers.
PG  - 236-9
AB  - Conventional and molecular cytogenetic analyses of three murine cancer cell lines 
      that had been induced in male athymic mice by the injection of three different 
      human prostate cancer cell lines revealed selective amplification of the Y 
      chromosome. In particular, analysis of metaphase and interphase nuclei by 
      fluorescence in situ hybridization (FISH) with the mouse Y chromosome-specific 
      DNA painting probe revealed the presence of various numbers of Y chromosomes, 
      ranging from one to eight, with a large majority of nuclei showing two copies 
      (46.5-60.1%). In Interphase nuclei, the Y chromosomes showed distinct morphology, 
      allowing identification irrespective of whether the preparations were treated for 
      15 min or for 5 h with Colcemid, a chemical known to cause chromosome 
      condensation. However, FISH performed on human lymphocyte cultures with 
      chromosome-specific DNA painting probes other than the Y chromosome did not 
      reveal condensed chromosome morphology in interphase nuclei even after 12 h of 
      Colcemid treatment. Our FISH results indicate that (1) the Y chromosome is 
      selectively amplified in all three cell lines; (2) the mouse Y chromosome number 
      is comparable in both interphase and metaphase cells: (3) the Y chromosome number 
      varies between one and eight, with a large majority of cells showing two or three 
      copies in most interphase nuclei; (4) the condensation of the Y chromosome is not 
      affected by the duration of Colcemid treatment but by its inherent DNA 
      constitution; and (5) the number of copies of the Y chromosome is increased and 
      retained not only in human prostate tumor cell lines but also in murine tumors 
      induced by these prostate tumor cell lines.
FAU - Multani, A S
AU  - Multani AS
AD  - Department of Cancer Biology, The University of Texas M.D. Anderson Cancer 
      Center, Houston 77030, USA.
FAU - Ozen, M
AU  - Ozen M
FAU - Agrawal, A
AU  - Agrawal A
FAU - Hopwood, V L
AU  - Hopwood VL
FAU - von Eschenbach, A C
AU  - von Eschenbach AC
FAU - Pathak, S
AU  - Pathak S
LA  - eng
GR  - RRO 4999-01/RR/NCRR NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - Germany
TA  - In Vitro Cell Dev Biol Anim
JT  - In vitro cellular & developmental biology. Animal
JID - 9418515
SB  - IM
MH  - Animals
MH  - *Cell Transformation, Neoplastic
MH  - Gene Amplification
MH  - Humans
MH  - Male
MH  - Mice
MH  - Mice, Nude
MH  - Prostatic Neoplasms/*genetics
MH  - Tumor Cells, Cultured
MH  - *Y Chromosome
EDAT- 1999/09/09 00:00
MHDA- 1999/09/09 00:01
CRDT- 1999/09/09 00:00
PHST- 1999/09/09 00:00 [pubmed]
PHST- 1999/09/09 00:01 [medline]
PHST- 1999/09/09 00:00 [entrez]
AID - 10.1007/s11626-999-0032-6 [doi]
PST - ppublish
SO  - In Vitro Cell Dev Biol Anim. 1999 Apr;35(4):236-9. doi: 
      10.1007/s11626-999-0032-6.