PMID- 10479390
OWN - NLM
STAT- MEDLINE
DCOM- 20001019
LR  - 20061115
IS  - 0896-8411 (Print)
IS  - 0896-8411 (Linking)
VI  - 13
IP  - 2
DP  - 1999 Sep
TI  - Isolation and characterization of two human monoclonal anti-phospholipid IgG from 
      patients with autoimmune disease.
PG  - 215-23
AB  - The antigenic specificity of anti-phospholipid antibodies (APA) is a matter of 
      intensive investigation. To further characterize these antibodies, we attempted 
      to isolate human monoclonal APA. B-cells of patients with at least one positive 
      test for antibodies against cardiolipin, phosphatidylserine, beta2-glycoprotein I 
      (beta2-GPI) or the lupus anti-coagulant were immortalized by transformation with 
      Epstein-Barr virus and screened for production of specific IgG. Positive pools 
      were fused with a heteromyeloma cell line and APA-secreting clones were isolated 
      by standard procedures. Two monoclonal APA, HL-5B from a 51-year-old man with 
      primary anti-phospholipid syndrome and recurrent cerebral microinfarctions, and 
      RR-7F from a 48-year-old women with systemic lupus erythematosus but no evidence 
      for thrombotic events were obtained. HL-5B is of the IgG2 subtype with lambda 
      light chains, while RR-7F is IgG2 with kappa light chains. Both monoclonals show 
      reactivity against cardiolipin and phosphatidylserine but lack reactivity against 
      beta2-GPI or lupus anti-coagulant activity. To yield the same OD in the 
      cardiolipin and phosphatidylserine ELISAs RR-7F must be used in an approximately 
      10-fold higher concentration than HL-5B, indicating a lower affinity towards 
      these antigens. Interestingly, both mAPA can bind to cardiolipin in the absence 
      of beta2-GPI. They do not cross-react with dsDNA but show reactivity against 
      oxidized low-density lipoproteins. Analysis of the heavy chain mRNA of HL-5B and 
      RR-7F showed that both are members of the VH3 family. While HL-5B shows extensive 
      somatic mutations in the CDR1 and 2 regions, indicating that it was derived by a 
      T cell-dependent antigen driven process, RR-7F is apparently germline encoded. 
      The two monoclonal APA can be used as tools in further structural and functional 
      analyses.
CI  - Copyright 1999 Academic Press.
FAU - von Landenberg, C
AU  - von Landenberg C
AD  - Institute for Clinical Chemistry and Laboratory Medicine, University Hospital of 
      Regensburg, Germany.
FAU - Lackner, K J
AU  - Lackner KJ
FAU - von Landenberg, P
AU  - von Landenberg P
FAU - Lang, B
AU  - Lang B
FAU - Schmitz, G
AU  - Schmitz G
LA  - eng
PT  - Case Reports
PT  - Journal Article
PL  - England
TA  - J Autoimmun
JT  - Journal of autoimmunity
JID - 8812164
RN  - 0 (Antibodies, Monoclonal)
RN  - 0 (Autoantibodies)
RN  - 0 (Cardiolipins)
RN  - 0 (Glycoproteins)
RN  - 0 (Immunoglobulin G)
RN  - 0 (Immunoglobulin Heavy Chains)
RN  - 0 (Immunoglobulin Variable Region)
RN  - 0 (Lupus Coagulation Inhibitor)
RN  - 0 (Phosphatidylserines)
RN  - 0 (Phospholipids)
RN  - 0 (beta 2-Glycoprotein I)
SB  - IM
MH  - Amino Acid Sequence
MH  - Antibodies, Monoclonal/*immunology
MH  - Antibody Specificity
MH  - Antiphospholipid Syndrome/*immunology
MH  - Autoantibodies/*immunology
MH  - Base Sequence
MH  - Cardiolipins/immunology
MH  - Female
MH  - Glycoproteins/immunology
MH  - Humans
MH  - Immunoglobulin G/genetics/*immunology
MH  - Immunoglobulin Heavy Chains/genetics
MH  - Immunoglobulin Variable Region/genetics
MH  - Lupus Coagulation Inhibitor/analysis
MH  - Male
MH  - Middle Aged
MH  - Molecular Sequence Data
MH  - Phosphatidylserines/immunology
MH  - Phospholipids/immunology
MH  - beta 2-Glycoprotein I
EDAT- 1999/09/10 09:00
MHDA- 2000/10/21 11:01
CRDT- 1999/09/10 09:00
PHST- 1999/09/10 09:00 [pubmed]
PHST- 2000/10/21 11:01 [medline]
PHST- 1999/09/10 09:00 [entrez]
AID - S0896-8411(99)90316-5 [pii]
AID - 10.1006/jaut.1999.0316 [doi]
PST - ppublish
SO  - J Autoimmun. 1999 Sep;13(2):215-23. doi: 10.1006/jaut.1999.0316.